验证数据展示
经过测试的应用
| Positive WB detected in | mouse colon tissue, mouse spleen tissue |
| Positive IHC detected in | mouse colon tissue, human pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IP | See 1 publications below |
产品信息
12911-1-AP targets AOAH in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IP |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag3892 Product name: Recombinant human AOAH protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 226-575 aa of BC025698 Sequence: QDSNCNGIWGVDPKDGVPYEKKFCEGSQPRGIILLGDSAGAHFHISPEWITASQMSLNSFINLPTALTNELDWPQLSGATGFLDSTVGIKEKSIYLRLWKRNHCNHRDYQNISRNGASSRNLKKFIESLSRNKVLDYPAIVIYAMIGNDVCSGKSDPVPAMTTPEKLYSNVMQTLKHLNSHLPNGSHVILYGLPDGTFLWDNLHNRYHPLGQLNKDMTYAQLYSFLNCLQVSPCHGWMSSNKTLRTLTSERAEQLSNTLKKIAASEKFTNFNLFYMDFAFHEIIQEWQKRGGQPWQLIEPVDGFHPNEVALLLLADHFWKKVQLQWPQILGKENPFNPQIKQVFGDQGGH 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | acyloxyacyl hydrolase (neutrophil) |
| 别名 | Acyloxyacyl hydrolase, Acyloxyacyl hydrolase large subunit, Acyloxyacyl hydrolase small subunit, EC:3.1.1.77 |
| 计算分子量 | 65 kDa |
| 观测分子量 | 65 kDa |
| GenBank蛋白编号 | BC025698 |
| 基因名称 | AOAH |
| Gene ID (NCBI) | 313 |
| RRID | AB_2877891 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P28039 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Acyloxyacyl hydrolase (AOAH) is a highly conserved lipase responsible for hydrolyzing the secondary fatty acyl chains of gram-negative bacterial lipopolysaccharides (LPS), resulting in LPS detoxification and attenuation of host inflammation (PMID: 1577781, PMID: 35980963). AOAH has also been shown to have PLA2 function (PMID: 1577781). AOAH was highly expressed at the protein level in lesional psoriatic skin compared to healthy controls (PMID: 34913478). AOAH has been described to be differentially proteolytically processed into both a secreted and cytosolic form in phagocytic cells (PMID: 19755182). Expression of AOAH is primarily attributed to phagocytic cells such as neutrophils, macrophages, DC, and also NK cells (PMID: 33454018).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for AOAH antibody 12911-1-AP | Download protocol |
| IHC protocol for AOAH antibody 12911-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
