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验证数据展示

  • WB analysis using 15416-1-AP

    WB analysis using 15416-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15416-1-AP (ANXA9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 15416-1-AP

    IHC staining of human breast cancer using 15416-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15416-1-AP (ANXA9 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 15416-1-AP

    IHC staining of human breast cancer using 15416-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15416-1-AP (ANXA9 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ANXA9 Polyclonal antibody

ANXA9 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 15416-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

Annexin 31, Annexin 9, annexin A9, Annexin XXXI, ANX31, ANXA9, Pemphaxin

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA431 cells, MCF-7 cells
Positive IHC detected inhuman breast cancer tissue, human colon cancer tissue, human heart tissue, human kidney tissue, human liver tissue, human placenta tissue, human skin tissue, human spleen tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

15416-1-AP targets ANXA9 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性human
免疫原

CatNo: Ag7702

Product name: Recombinant human ANXA9 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-347 aa of BC005830

Sequence: STMSVTGGKMAPSLTQEILSHLGLASKTAAWGTLGTLRTFLNFSVDKDAQRLLRAITGQGVDRSAIVDVLTNRSREQRQLISRNFQERTQQDLMKSLQAALSGNLERIVMALLQPTAQFDAQELRTALKASDSAVDVAIEILATRTPPQLQECLAVYKHNFQVEAVDDITSETSGILQDLLLALAKGGRDSYSGIIDYNLAEQDVQALQRAEGPSREETWVPVFTQRNPEHLIRVFDQYQRSTGQELEEAVQNRFHGDAQVALLGLASVIKNTPLYFADKLHQALQETEPNYQVLIRILISRCETDLLSIRAEFRKKFGKSLYSSLQDAVKGDCQSALLALCRAEDM

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 annexin A9
别名 Annexin 31, Annexin 9, annexin A9, Annexin XXXI, ANX31, ANXA9, Pemphaxin
计算分子量 38 kDa
观测分子量 38 kDa
GenBank蛋白编号BC005830
基因名称 ANXA9
Gene ID (NCBI) 8416
RRIDAB_2057905
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO76027
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ANXA9 antibody 15416-1-APDownload protocol
IHC protocol for ANXA9 antibody 15416-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Biol Reprod

Rictor stability mediated by USP9X regulates embryo implantation by participating in lipid metabolism of endometrium

Authors - Mingyu Peng
View Article