验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, A549 cells, human brain tissue, Jurkat cells, mouse brain tissue, mouse kidney tissue, mouse placenta tissue |
| Positive IP detected in | HeLa cells, mouse kidney tissue |
| Positive IHC detected in | human colon tissue, human kidney tissue, human normal colon, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12542-1-AP targets Annexin VI in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag3263 Product name: Recombinant human ANXA6 protein Source: e coli.-derived, T-HIS Tag: 6*His Domain: 324-674 aa of BC017046 Sequence: DDDAAGQFFPEAAQVAYQMWELSAVARVELKGTVRPANDFNPDADAKALRKAMKGLGTDEDTIIDIITHRSNVQRQQIRQTFKSHFGRDLMTDLKSEISGDLARLILGLMMPPAHYDAKQLKKAMEGAGTDEKALIEILATRTNAEIRAINEAYKEDYHKSLEDALSSDTSGHFRRILISLATGHREEGGENLDQAREDAQVAAEILEIADTPSGDKTSLETRFMTILCTRSYPHLRRVFQEFIKMTNYDVEHTIKKEMSGDVRDAFVAIVQSVKNKPLFFADKLYKSMKGAGTDEKTLTRIMVSRSEIDLLNIRREFIEKYDKSLHQAIEGDTSGDFLKALLALCGGED 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | annexin A6 |
| 别名 | Annexin A6, ANXA6, 67 kDa calelectrin, Annexin 6, Annexin-6 |
| 计算分子量 | 673 aa, 76 kDa |
| 观测分子量 | 67 kDa |
| GenBank蛋白编号 | BC017046 |
| 基因名称 | Annexin VI |
| Gene ID (NCBI) | 309 |
| RRID | AB_2057753 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P08133 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ANXA6, also called CBP68, or 67 kDa calelectrin, belongs to a family of calcium-dependent membrane and phospholipid binding proteins. Several members of the annexin family have been implicated in membrane-related events along exocytotic and endocytotic pathways. The annexin VI gene is approximately 60 kbp long and contains 26 exons. It encodes a protein of about 68 kDa that consists of eight 68-amino acid repeats separated by linking sequences of variable lengths. It is highly similar to human annexins I and II sequences, each of which contain four such repeats. Annexin VI has been implicated in mediating the endosome aggregation and vesicle fusion in secreting epithelia during exocytosis.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Annexin VI antibody 12542-1-AP | Download protocol |
| IHC protocol for Annexin VI antibody 12542-1-AP | Download protocol |
| IF protocol for Annexin VI antibody 12542-1-AP | Download protocol |
| IP protocol for Annexin VI antibody 12542-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) Isolation and Comprehensive Analysis of Cochlear Tissue-Derived Small Extracellular Vesicles | ||
J Extracell Vesicles Distinguishing functional exosomes and other extracellular vesicles as a nucleic acid cargo by the anion-exchange method. | ||
Oncotarget CD300A promotes tumor progression by PECAM1, ADCY7 and AKT pathway in acute myeloid leukemia. | ||
Front Cell Dev Biol Dynamic Mitochondrial Proteome Under Polyamines Treatment in Cardiac Aging. | ||
Proteome Sci A dynamic plasma membrane proteome analysis of alcohol-induced liver cirrhosis. |
