验证数据展示

WB analysis using 60051-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 60051-1-Ig (Annexin A2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.
WB analysis of THP-1 using 60051-1-Ig
WB result of Annexin A2 antibody (60051-1-Ig; 1:10000; room temperature for 1.5 hours) with wild-type and sg-Annexin A2 transfected THP-1 cells.
WB analysis of HEK-293 using 60051-1-Ig
WB result of Annexin A2 antibody (60051-1-Ig, 1:20,000) with si-Control and si-Annexin A2 transfected HEK293 cells.
IHC staining of human breast cancer using 60051-1-Ig
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60051-1-Ig (Annexin A2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with ANXA2 antibody included
IHC staining of human breast cancer using 60051-1-Ig
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60051-1-Ig (Annexin A2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with ANXA2 antibody included
IF Staining of T-47D using 60051-1-Ig
Immunofluorescent analysis of (-20°C Ethanol) fixed T-47D cells using 60051-1-Ig (Annexin A2 antibody), at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
IP experiment of HeLa using 60051-1-Ig
IP result of anti-Annexin A2 (IP:60051-1-Ig, 4ug; Detection:60051-1-Ig 1:1500) with HeLa cells lysate 2400ug.
IF Staining of MCF-7 using 60051-1-Ig
Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using Annexin A2 antibody (60051-1-Ig, Clone: 3H1B11 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

Featured Product
KD/KO验证

Annexin A2 Monoclonal antibody

Annexin A2 Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 60051-1-Ig

产品说明书

CloneNo. 3H1B11

引用文献(11)

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA and More (2)

别名

ANXA2, 3H1B11, Annexin 2, Annexin II, Annexin-2

缓冲液配方: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

偶联物: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite®594

规格	价格	库存

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概述

经过测试的应用
产品信息
发表文章 (11)
实验方案

产品说明书

MSDS

经过测试的应用

Positive WB detected in	K-562 cells, HEK-293 cells, THP-1 cells, A431 cells, A549 cells, HeLa cells, HepG2 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human breast cancer tissue, human lung cancer tissue, human skin cancer tissue, human gliomas tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	T-47D cells, HeLa cells, MCF-7 cells

Planning an IHC experiment? We recommend our IHCeasy ANXA2 Ready-To-Use IHC Kit. ANXA2 primary antibody included.

Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

应用	推荐稀释比
Western Blot (WB)	WB : 1:5000-1:20000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 2 publications below
WB	See 8 publications below
IF	See 2 publications below
IP	See 3 publications below
CoIP	See 1 publications below

产品信息

60051-1-Ig targets Annexin A2 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用	WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用	WB, IF, IP, CoIP
经测试反应性	human, mouse, rat
文献引用反应性	human, mouse
免疫原	CatNo: Ag1777 Product name: Recombinant human Annexin A2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-339 aa of BC016774 Sequence: MSTVHEILCKLSLEGDHSTPPSAYGSVKAYTNFDAERDALNIETAIKTKGVDEVTIVNILTNRSNAQRQDIAFAYQRRTKKELASALKSALSGHLETVILGLLKTPAQYDASELKASMKGLGTDEDSLIEIICSRTNQELQEINRVYKEMYKTDLEKDIISDTSGDFRKLMVALAKGRRAEDGSVIDYELIDQDARDLYDAGVKRKGTDVPKWISIMTERSVPHLQKVFDRYKSYSPYDMLESIRKEVKGDLENAFLNLVQCIQNKPLYFADRLYDSMKGKGTRDKVLIRIMVSRSEVDMLKIRSEFKRKYGKSLYYYIQQDTKGDYQKALLYLCGGDD 种属同源性预测
宿主/亚型	Mouse / IgG2b
抗体类别	Monoclonal
产品类型	Antibody
全称	annexin A2
别名	ANXA2, 3H1B11, Annexin 2, Annexin II, Annexin-2
计算分子量	339 aa, 39 kDa
观测分子量	35-38 kDa
GenBank蛋白编号	BC016774
基因名称	Annexin A2
Gene ID (NCBI)	302
ENSEMBL Gene ID	ENSG00000182718
RRID	AB_2057309
偶联类型	Unconjugated
形式	Liquid
纯化方式	Protein A purification
UNIPROT ID	P07355
储存缓冲液	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

Annexin A2 (ANXA2), also named Annexin-2 or Lipocortin II, is a Ca2+ binding protein that is up-regulated in virally transformed cell lines and human tumors. This calcium-regulated membrane-binding protein, whose affinity for calcium is greatly enhanced by anionic phospholipids, may cross-link plasma membrane phospholipids with actin and the cytoskeleton and be involved with exocytosis. Heat-stress response may also involve Annexin-2.

实验方案

Product Specific Protocols
IF protocol for Annexin A2 antibody 60051-1-Ig	Download protocol
IHC protocol for Annexin A2 antibody 60051-1-Ig	Download protocol
IP protocol for Annexin A2 antibody 60051-1-Ig	Download protocol
WB protocol for Annexin A2 antibody 60051-1-Ig	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
mouse	WB,CoIP	Cell Streptococcus anginosus promotes gastric inflammation, atrophy, and tumorigenesis in mice Authors - Kaili Fu View Article
human	IP	EBioMedicine Compounds targeting YadC of uropathogenic Escherichia coli and its host receptor annexin A2 decrease bacterial colonization in bladder. Authors - Xiao Li View Article
		Infect Immun The Tick Protein Sialostatin L2 Binds to Annexin A2 and Inhibits NLRC4-Mediated Inflammasome Activation. Authors - Xiaowei Wang View Article
mouse	WB	Cell Mol Life Sci Upregulation of HDAC9 in hippocampal neurons mediates depression-like behaviours by inhibiting ANXA2 degradation Authors - Yunjian Dai View Article
mouse,human	WB,IP,IF	iScience Opportunistic pathogen Porphyromonas gingivalis targets the LC3B-ceramide complex and mediates lethal mitophagy resistance in oral tumors Authors - Megan Sheridan KD Validated View Article
human	WB,IF	Cell Signal The novel DNA methylation marker FIBIN suppresses non-small cell lung cancer metastasis by negatively regulating ANXA2 Authors - Mingyu Peng View Article

验证数据展示

WB analysis using 60051-1-Ig

WB analysis of THP-1 using 60051-1-Ig

WB analysis of HEK-293 using 60051-1-Ig

IHC staining of human breast cancer using 60051-1-Ig

IHC staining of human breast cancer using 60051-1-Ig

IF Staining of T-47D using 60051-1-Ig

IP experiment of HeLa using 60051-1-Ig

IF Staining of MCF-7 using 60051-1-Ig

Annexin A2 Monoclonal antibody

Annexin A2 Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

Streptococcus anginosus promotes gastric inflammation, atrophy, and tumorigenesis in mice

Compounds targeting YadC of uropathogenic Escherichia coli and its host receptor annexin A2 decrease bacterial colonization in bladder.

The Tick Protein Sialostatin L2 Binds to Annexin A2 and Inhibits NLRC4-Mediated Inflammasome Activation.

Upregulation of HDAC9 in hippocampal neurons mediates depression-like behaviours by inhibiting ANXA2 degradation

Opportunistic pathogen Porphyromonas gingivalis targets the LC3B-ceramide complex and mediates lethal mitophagy resistance in oral tumors

The novel DNA methylation marker FIBIN suppresses non-small cell lung cancer metastasis by negatively regulating ANXA2