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Annexin A2 Monoclonal antibody
Annexin A2 Monoclonal Antibody for FC, IF/ICC, IHC, IP, WB, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
human, mouse, rat
Applications
FC, IF/ICC, IHC, IP, WB, ELISA
Conjugate
Unconjugated
CloneNo.
3H1B11
验证数据展示
经过测试的应用
Positive WB detected in | K-562 cells, HEK-293 cells, A431 cells, A549 cells, HeLa cells, HepG2 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human breast cancer tissue, human lung cancer tissue, human skin cancer tissue, human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | T-47D cells, Hela cells, MCF-7 cells |
Positive FC detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:20000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:250-1:1000 |
Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 1 publications below |
IP | See 1 publications below |
产品信息
60051-1-Ig targets Annexin A2 in WB, IP, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | FC, IF/ICC, IHC, IP, WB, ELISA |
Cited Applications | WB, IP |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Immunogen | Annexin A2 fusion protein Ag1777 种属同源性预测 |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Full Name | annexin A2 |
Synonyms | Annexin 2, annexin A2, Annexin II, ANX2, ANX2L4, ANXA2, CAL1H, Calpactin 1 heavy chain, Calpactin I heavy chain, Chromobindin 8, LIP2, Lipocortin II, LPC2, LPC2D, P36, PAP IV, Protein I |
Calculated Molecular Weight | 339 aa, 39 kDa |
Observed Molecular Weight | 35-38 kDa |
GenBank Accession Number | BC016774 |
Gene Symbol | ANXA2 |
Gene ID (NCBI) | 302 |
ENSEMBL Gene ID | ENSG00000182718 |
RRID | AB_2057309 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P07355 |
Storage Buffer | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for Annexin A2 antibody 60051-1-Ig | Download protocol |
IHC protocol for Annexin A2 antibody 60051-1-Ig | Download protocol |
IF protocol for Annexin A2 antibody 60051-1-Ig | Download protocol |
IP protocol for Annexin A2 antibody 60051-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
EBioMedicine Compounds targeting YadC of uropathogenic Escherichia coli and its host receptor annexin A2 decrease bacterial colonization in bladder. | ||
Infect Immun The Tick Protein Sialostatin L2 Binds to Annexin A2 and Inhibits NLRC4-Mediated Inflammasome Activation. | ||
Cell Mol Life Sci Upregulation of HDAC9 in hippocampal neurons mediates depression-like behaviours by inhibiting ANXA2 degradation |