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验证数据展示

  • WB analysis using 11256-1-AP

    WB analysis using 11256-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11256-1-AP (Annexin A2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 11256-1-AP

    WB analysis of HEK-293 using 11256-1-AP

    WB result of Annexin A2 (11256-1-AP, 1:1000) with si-Control and si-Annexin A2 transfected HEK293 cells.

  • WB analysis using 11256-1-AP

    WB analysis using 11256-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11256-1-AP (Annexin A2 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 11256-1-AP

    IHC staining of human liver using 11256-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11256-1-AP (Annexin A2 Antibody) at dilution of 1:200 (under 10x lens).

    Ready-to-use IHC kits available with ANXA2 antibody included
  • IHC staining of human liver using 11256-1-AP

    IHC staining of human liver using 11256-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11256-1-AP (Annexin A2 Antibody) at dilution of 1:200 (under 40x lens).

    Ready-to-use IHC kits available with ANXA2 antibody included
  • FC experiment of K-562 using 11256-1-AP

    FC experiment of K-562 using 11256-1-AP

    1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human Annexin A2 (11256-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • WB analysis of THP-1 using 11256-1-AP

    WB analysis of THP-1 using 11256-1-AP

    WB result of Annexin A2 antibody (11256-1-AP; 1:20000; room temperature for 1.5 hours) with wild-type and sg-Annexin A2 transfected THP-1 cells.

  • IHC staining of mouse liver using 11256-1-AP

    IHC staining of mouse liver using 11256-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 11256-1-AP (Annexin A2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ANXA2 antibody included
  • IHC staining of mouse liver using 11256-1-AP

    IHC staining of mouse liver using 11256-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 11256-1-AP (Annexin A2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ANXA2 antibody included
  • FC experiment of A549 using 11256-1-AP

    FC experiment of A549 using 11256-1-AP

    1x10^6 A549 cells were intracellularly stained with 0.25 ug Annexin A2 Polyclonal antibody (11256-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IP experiment of HeLa using 11256-1-AP

    IP experiment of HeLa using 11256-1-AP

    IP result of anti-Annexin A2 (IP:11256-1-AP, 4ug; Detection:11256-1-AP 1:3000) with HeLa cells lysate 2640 ug.

  • IHC staining of mouse kidney using 11256-1-AP

    IHC staining of mouse kidney using 11256-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11256-1-AP (Annexin A2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ANXA2 antibody included
  • IHC staining of mouse liver using 11256-1-AP

    IHC staining of mouse liver using 11256-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 11256-1-AP (Annexin A2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ANXA2 antibody included
  • IHC staining of mouse placenta using 11256-1-AP

    IHC staining of mouse placenta using 11256-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 11256-1-AP (Annexin A2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ANXA2 antibody included
  • IHC staining of mouse placenta using 11256-1-AP

    IHC staining of mouse placenta using 11256-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 11256-1-AP (Annexin A2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ANXA2 antibody included
  • IHC staining of mouse placenta using 11256-1-AP

    IHC staining of mouse placenta using 11256-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 11256-1-AP (Annexin A2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ANXA2 antibody included
  • IHC staining of human bowen disease using 11256-1-AP

    IHC staining of human bowen disease using 11256-1-AP

    Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 11256-1-AP (Annexin A2 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ANXA2 antibody included
  • IHC staining of human bowen disease using 11256-1-AP

    IHC staining of human bowen disease using 11256-1-AP

    Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 11256-1-AP (Annexin A2 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ANXA2 antibody included
  • IF Staining of MCF-7 using 11256-1-AP

    IF Staining of MCF-7 using 11256-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using Annexin A2 antibody (11256-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  • IF Staining of HeLa using 11256-1-AP

    IF Staining of HeLa using 11256-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Annexin A2 antibody (11256-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

  • Featured Product
  • KD/KO验证

Annexin A2 Polyclonal antibody

Annexin A2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Cat No. 11256-1-AP

产品说明书

引用文献(72)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (5)

应用

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (3)

别名

ANXA2, Annexin 2, Annexin II, Annexin-2, ANX2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, mouse placenta tissue, THP-1 cells, NIH/3T3 cells, mouse kidney tissue, rat kidney tissue, HepG2 cells
Positive IP detected inHeLa cells
Positive IHC detected inmouse placenta tissue, human bowen disease tissue, human breast cancer tissue, human hepatocirrhosis tissue, human liver tissue, human lung cancer tissue, human oesophagus cancer tissue, human prostate cancer tissue, mouse kidney tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, MCF-7 cells
Positive FC (Intra) detected inK-562 cells, A549 cells
Planning an IHC experiment? We recommend our IHCeasy ANXA2 Ready-To-Use IHC Kit. ANXA2 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11256-1-AP targets Annexin A2 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP, CoIP, RIP, ELISA
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat, pig, monkey, chicken, zebrafish, bovine
免疫原 Annexin A2 fusion protein Ag1777 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 annexin A2
别名 ANXA2, Annexin 2, Annexin II, Annexin-2, ANX2
计算分子量 339 aa, 39 kDa
观测分子量 35-40 kDa
GenBank蛋白编号BC016774
基因名称 Annexin A2
Gene ID (NCBI) 302
ENSEMBL Gene IDENSG00000182718
RRIDAB_2057311
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP07355
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Annexin A2 (ANXA2), also named Annexin-2 or Lipocortin II, is a Ca2+ binding protein that is up-regulated in virally transformed cell lines and human tumors. This calcium-regulated membrane-binding protein, whose affinity for calcium is greatly enhanced by anionic phospholipids, may cross-link plasma membrane phospholipids with actin and the cytoskeleton and be involved with exocytosis. Heat-stress response may also involve Annexin-2.

实验方案

Product Specific Protocols
WB protocol for Annexin A2 antibody 11256-1-APDownload protocol
IHC protocol for Annexin A2 antibody 11256-1-APDownload protocol
IF protocol for Annexin A2 antibody 11256-1-APDownload protocol
IP protocol for Annexin A2 antibody 11256-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human

Nat Immunol

NKILA lncRNA promotes tumor immune evasion by sensitizing T cells to activation-induced cell death.

Authors - Di Huang
View Article
mouseWB

Adv Sci (Weinh)

Isolation and Comprehensive Analysis of Cochlear Tissue-Derived Small Extracellular Vesicles

Authors - Pei Jiang
View Article
humanWB

Sci Adv

p53 loss activates prometastatic secretory vesicle biogenesis in the Golgi.

Authors - Xiaochao Tan
View Article
humanWB

Nat Chem Biol

YAP-dependent proliferation by a small molecule targeting annexin A2.

Authors - Sophia Z Shalhout
  • KD Validated
View Article
humanWB

Nat Commun

PMID: 31792210

Authors - Sunwang Xu
View Article
mouseWB

Nucleic Acids Res

Short DNA/RNA heteroduplex oligonucleotide interacting proteins are key regulators of target gene silencing.

Authors - Ken Asada
View Article