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验证数据展示

  • WB analysis using 17478-1-AP (same clone as 17478-1-PBS)

    WB analysis using 17478-1-AP (same clone as 17478-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 17478-1-AP (ANUBL1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17478-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 17478-1-AP (same clone as 17478-1-PBS)

    IHC staining of human stomach cancer using 17478-1-AP (same clone as 17478-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 17478-1-AP (ANUBL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17478-1-PBS in a different storage buffer formulation.

ANUBL1 Polyclonal antibody, PBS Only

ANUBL1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 17478-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

17478-1-PBS targets ANUBL1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag11505

Product name: Recombinant human ANUBL1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 305-653 aa of BC048968

Sequence: GNIVLPSEECVTEQSLLPKVGSLASFAEGNADEQSSGLEGACKVNLELLLTNADKGLKAPEQHLKHVAGVLNGESVETSVLNYRELSPHKNRLLSPLRCSAPMSLHNSLVKPERQSKCFEFGKLQPSSSQSLDVQNITDSSFSRTTCFQGVKVDSLGKRSDVISKVEARDITEMTNKASKEPVGCVNNISFLASLAGSTSRNRLQSTRGAGRLQNSGTGLSTNLQHFQEENFRKSSPQLEHTGVFLSTHGVGMNGNNAAAGKSVGECTTHHLPPVKAPLQTKKKTTNHCFLCGKKTGLASSYECRCGNNFCASHRYAETHGCTYDYKSAGRRYLHEANPVVNAPKLPKI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 AN1, ubiquitin-like, homolog (Xenopus laevis)
别名
计算分子量 653 aa, 72 kDa
观测分子量70-80 kDa
GenBank蛋白编号BC048968
基因名称 ANUBL1
Gene ID (NCBI) 93550
RRIDAB_2923568
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ86XD8
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ANUBL1 is also named as ZFAND4. The expression of ANUBL1 was specifically upregulated in gastric cancer and it was positively associated with grades of gastric cancer. Moreover, it promoted proliferation of gastric cancer SGC-7901 cells. The mechanism of ANUBL1 upregulation in gastric cancer is that miR-182 suppressed the expression of ANUBL1 through targeting its 3'UTR, and ANUBL1 could downregulate the expression of anti-proliferation-associated miRNAs (miR-148b, miR-375 and miR-182) in SGC-7901 cells. The calculated molecular weight of ANUBL1 is 80 kDa (PMID: 25682742).

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