CD13 Polyclonal antibody
CD13 Polyclonal Antibody for FC, IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (4)
Applications
FC, IF, IHC, WB, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HepG2 cells, human liver tissue, human kidney tissue, PC-3 cells, THP-1 cells, mouse kidney tissue, mouse liver tissue |
Positive IHC detected in | human tonsillitis tissue, human colon tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | mouse kidney tissue |
Positive FC detected in | HEK-293 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:8000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
14553-1-AP targets CD13 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | FC, IF, IHC, WB, ELISA |
Cited Applications | IF, IHC, IP, WB |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, monkey, rabbit, pig, canine |
Immunogen | CD13 fusion protein Ag5976 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | alanyl (membrane) aminopeptidase |
Synonyms | Alanyl aminopeptidase, Aminopeptidase M, Aminopeptidase N, ANPEP, AP M, AP N, APN, CD13, gp150, hAPN, LAP1, Microsomal aminopeptidase, p150, PEPN |
Calculated Molecular Weight | 110 kDa |
Observed Molecular Weight | 150 kDa |
GenBank Accession Number | BC058928 |
Gene Symbol | ANPEP |
Gene ID (NCBI) | 290 |
ENSEMBL Gene ID | ENSG00000166825 |
RRID | AB_2056591 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P15144 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for CD13 antibody 14553-1-AP | Download protocol |
IHC protocol for CD13 antibody 14553-1-AP | Download protocol |
IF protocol for CD13 antibody 14553-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Mol Cancer Circular RNA circIPO11 drives self-renewal of liver cancer initiating cells via Hedgehog signaling. | ||
Anal Chem Rapid Screening of Peptide Probes through in situ Single Bead Sequencing Microarray. | ||
Neuropathol Appl Neurobiol Brain mural cell loss in the parietal cortex in Alzheimer's disease correlates with cognitive decline and TDP-43 pathology. | ||
Front Oncol YBX1 Enhances Metastasis and Stemness by Transcriptionally Regulating MUC1 in Lung Adenocarcinoma. | ||
PLoS Negl Trop Dis Proteomic Analysis of Urine Exosomes Reveals Renal Tubule Response to Leptospiral Colonization in Experimentally Infected Rats. | ||
Exp Cell Res siRNA knockdown of alanine aminopeptidase impairs myoblast proliferation and differentiation. |