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验证数据展示

  • Sandwich ELISA standard curve of MP50363-3

    Sandwich ELISA standard curve of MP50363-3

    Sandwich ELISA standard curve of MP50363-3, Human ANP32B Monoclonal Matched Antibody Pair - PBS only. 66160-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag1274. 66160-2-PBS was HRP conjugated as the detection antibody. Range: 19.5-1250 pg/mL

  • Sample test of MP50363-3

    Sample test of MP50363-3

    The mean ANP32B concentration was determined to be 105.34 ng/mL in HCT116 cell extract based on a 2.0 mg/mL extract load, 83.16 ng/mL in A431 cell extract based on a 1.3 mg/mL extract load and 84.78 ng/mL in HeLa cell extract based on a 1.5 mg/mL extract load.

  • Cytometric bead array standard curve of MP50363-1

    Cytometric bead array standard curve of MP50363-1

    Cytometric bead array standard curve of MP50363-1, ANP32B Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66160-1-PBS. Detection antibody: 66160-2-PBS. Standard:Ag1274. Range: 1.563-200 ng/mL.

  • Cytometric bead array standard curve of MP50363-3

    Cytometric bead array standard curve of MP50363-3

    Cytometric bead array standard curve of MP50363-3, ANP32B Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66160-3-PBS. Detection antibody: 66160-2-PBS. Standard:Ag1274. Range: 1.563-200 ng/mL.

ANP32B Monoclonal antibody, PBS Only (Detector)

ANP32B Monoclonal Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 66160-2-PBS

产品说明书

CloneNo. 3B6G9

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

别名

Silver-stainable protein SSP29, Putative HLA-DR-associated protein I-2, PHAPI2, APRIL, Acidic leucine-rich nuclear phosphoprotein 32 family member B

缓冲液配方:  PBS Only
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy ANP32B Ready-To-Use IHC Kit. ANP32B primary antibody included.

产品信息

66160-2-PBS targets ANP32B as part of a matched antibody pair:

MP50363-1: 66160-1-PBS capture and 66160-2-PBS detection (validated in Cytometric bead array)

MP50363-3: 66160-3-PBS capture and 66160-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human
免疫原

CatNo: Ag1274

Product name: Recombinant human ANP32B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-177 aa of BC013003

Sequence: MDMKRRIHLELRNRTPAAVRELVLDNCKSNDGKIEGLTAEFVNLEFLSLINVGLISVSNLPKLPKLKKLELSENRIFGGLDMLAEKLPNLTHLNLSGNKLKDISTLEPLKKLECLKSLDLFNCEVTNLNDYRESVFKLLPQLTYLDGYDREDQEAPDSDAEVDGVDEEEEDEEGEDEEDEDDEDGEEEEFDEEDDEDEDVEGDEDDDEVSEEEEEFGLDEEDEDEDEDEEEEEGGKGEKRKRETDDEGEDD

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 acidic (leucine-rich) nuclear phosphoprotein 32 family, member B
别名 Silver-stainable protein SSP29, Putative HLA-DR-associated protein I-2, PHAPI2, APRIL, Acidic leucine-rich nuclear phosphoprotein 32 family member B
计算分子量 29 kDa
GenBank蛋白编号BC013003
基因名称 ANP32B
Gene ID (NCBI) 10541
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ92688
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C