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- KD/KO Validated
ANP32B Monoclonal antibody
ANP32B Monoclonal Antibody for IF/ICC, WB, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
human, mouse, rat
Applications
IF/ICC, WB, ELISA
Conjugate
Unconjugated
CloneNo.
5A1F12
验证数据展示
经过测试的应用
| Positive WB detected in | LNCaP cells, fetal human brain tissue, HEK-293 cells, mouse brain tissue, U2OS cells, A431 cells, HCT 116 cells, HeLa cells |
| Positive IF detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy ANP32B Ready-To-Use IHC Kit. ANP32B primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
产品信息
66160-1-Ig targets ANP32B in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | IF/ICC, WB, ELISA |
| Cited Applications | WB |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Immunogen | ANP32B fusion protein Ag1274 种属同源性预测 |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | acidic (leucine-rich) nuclear phosphoprotein 32 family, member B |
| Synonyms | ANP32B, APRIL, PHAPI2, Silver stainable protein SSP29, SSP29 |
| Calculated Molecular Weight | 29 kDa |
| Observed Molecular Weight | 29 kDa |
| GenBank Accession Number | BC013003 |
| Gene Symbol | ANP32B |
| Gene ID (NCBI) | 10541 |
| RRID | AB_2881556 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q92688 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ANP32B is a member of the highly conserved acidic leucine-rich nuclear phosphoprotein 32 (ANP32) family. The ANP32 proteins have been implicated in a broad array of physiological processes, including cell differentiation, apoptotic cell death, and cell proliferation. ANP32B was identified as a novel direct substrate for caspase-3 and acted as a negative regulator for leukemic cell apoptosis. ANP32B controls the expression of the dendritic cell maturation factor CD83 by regulating the transport of its mRNA to the cytoplasm. It also modulates the activity of the transcription factor Kruppel-like factor 5 (KLF5).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ANP32B antibody 66160-1-Ig | Download protocol |
| IF protocol for ANP32B antibody 66160-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Virol ANP32 proteins are essential for influenza virus replication in human cells.
|
