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ANO3 Polyclonal antibody

ANO3 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

IP, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 19489-1-AP

Print datasheet

Synonyms

ANO3, anoctamin 3, C11orf25, GENX 3947, TMEM16C, Transmembrane protein 16C

验证数据展示

  • WB analysis using 19489-1-AP

    WB analysis using 19489-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19489-1-AP (ANO3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse testis using 19489-1-AP

    IP experiment of mouse testis using 19489-1-AP

    IP result of anti-ANO3 (IP:19489-1-AP, 4ug; Detection:19489-1-AP 1:1000) with mouse testis tissue lysate 4000ug.

经过测试的应用

Positive WB detected inmouse brain tissue, mouse skin tissue, mouse ovary tissue, mouse kidney tissue
Positive IP detected inmouse testis tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19489-1-AP targets ANO3 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IP, WB, ELISA
Cited ApplicationsIP, IF
Tested Reactivity human, mouse
Cited Reactivityrat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name anoctamin 3
Synonyms ANO3, anoctamin 3, C11orf25, GENX 3947, TMEM16C, Transmembrane protein 16C
Calculated Molecular Weight 115 kDa
Observed Molecular Weight 100-115 kDa
GenBank Accession NumberNM_031418
Gene Symbol ANO3
Gene ID (NCBI) 63982
RRIDAB_10638489
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BYT9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ANO3, also named as C11orf25, TMEM16C and GENX-3947, belongs to the anoctamin family. It may act as a calcium-activated chloride channel.

实验方案

Product Specific Protocols
WB protocol for ANO3 antibody 19489-1-APDownload protocol
IP protocol for ANO3 antibody 19489-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratIP, IF

Neurosci Bull

A Role for Transmembrane Protein 16C/Slack Impairment in Excitatory Nociceptive Synaptic Plasticity in the Pathogenesis of Remifentanil-induced Hyperalgesia in Rats.

Authors - Yize Li
View Article