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验证数据展示

  • WB analysis using 20647-1-AP (same clone as 20647-1-PBS)

    WB analysis using 20647-1-AP (same clone as 20647-1-PBS)

    Y79 cells were subjected to SDS PAGE followed by western blot with 20647-1-AP (ANO2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20647-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse eye using 20647-1-AP (same clone as 20647-1-PBS)

    IHC staining of mouse eye using 20647-1-AP (same clone as 20647-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 20647-1-AP (ANO2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20647-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse brain using 20647-1-AP (same clone as 20647-1-PBS)

    IP experiment of mouse brain using 20647-1-AP (same clone as 20647-1-PBS)

    IP result of anti-ANO2 (IP:20647-1-AP, 4ug; Detection:20647-1-AP 1:300) with mouse brain tissue lysate 10000ug. This data was developed using the same antibody clone with 20647-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse eye using 20647-1-AP (same clone as 20647-1-PBS)

    IHC staining of mouse eye using 20647-1-AP (same clone as 20647-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 20647-1-AP (ANO2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20647-1-PBS in a different storage buffer formulation.

  • IHC staining of rat eye using 20647-1-AP (same clone as 20647-1-PBS)

    IHC staining of rat eye using 20647-1-AP (same clone as 20647-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat eye tissue slide using 20647-1-AP (ANO2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20647-1-PBS in a different storage buffer formulation.

  • IF Staining of rat eye using 20647-1-AP (same clone as 20647-1-PBS)

    IF Staining of rat eye using 20647-1-AP (same clone as 20647-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat eye tissue using ANO2 antibody (20647-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20647-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

ANO2 Polyclonal antibody, PBS Only

ANO2 Polyclonal Antibody for WB, IHC, IF-P, IP, Indirect ELISA
Cat No. 20647-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, IP, Indirect ELISA

别名

anoctamin 2, Anoctamin-2, C12orf3, TMEM16B, Transmembrane protein 16B

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

20647-1-PBS targets ANO2 in WB, IHC, IF-P, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 anoctamin 2
别名 anoctamin 2, Anoctamin-2, C12orf3, TMEM16B, Transmembrane protein 16B
计算分子量 114 kDa
观测分子量 114 kDa
GenBank蛋白编号NM_020373
基因名称 ANO2
Gene ID (NCBI) 57101
RRIDAB_10696181
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NQ90
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ANO2, also named as C12orf3 and TMEM16B, belongs to the anoctamin family. It acts as a calcium-activated chloride channel (CaCC), mostly in photoreceptors. It may mediate olfactory amplification in olfactory sensory neurons (OSNs) and light perception amplification in retina.

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