验证数据展示
经过测试的应用
| Positive WB detected in | BxPC-3 cells, Caco-2 cells, HeLa cells |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
20447-1-AP targets ANKZF1 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag14285 Product name: Recombinant human ANKZF1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 377-726 aa of BC000238 Sequence: TEEEIRKICRDEKEALGQNEESPKQGSGSEGEDGFQVELELVELTVGTLDLCESEVLPKRRRRKRNKKEKSRDQEAGAHRTLLQQTQEEEPSTQSSQAVAAPLGPLLDEAKAPGQPELWNALLAACRAGDVGVLKLQLAPSPADPRVLSLLSAPLGSGGFTLLHAAAAAGRGSVVRLLLEAGADPTVQDSRARPPYTVAADKSTRNEFRRFMEKNPDAYDYNKAQVPGPLTPEMEARQATRKREQKAARRQREEQQQRQQEQEEREREEQRRFAALSDREKRALAAERRLAAQLGAPTSPIPDSAIVNTRRCWSCGASLQGLTPFHYLDFSFCSTRCLQDHRRQAGRPSS 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ankyrin repeat and zinc finger domain containing 1 |
| 别名 | Ankyrin repeat and zinc finger domain-containing protein 1, ANKZF 1, EC:3.1.-.-, tRNA endonuclease ANKZF1, ZNF744 |
| 计算分子量 | 726 aa, 81 kDa |
| 观测分子量 | 81 kDa |
| GenBank蛋白编号 | BC000238 |
| 基因名称 | ANKZF1 |
| Gene ID (NCBI) | 55139 |
| RRID | AB_2878688 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9H8Y5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ANKZF1, the mammalian ortholog of yeast Vms1, functions in the ribosome quality control (RQC) pathway as a tRNA hydrolase that recycles nascent peptides from stalled ribosomes, and it also counterbalances Rqc2‑mediated CAT‑tailing, thereby limiting the import of CAT‑tailed mitochondrial precursor proteins and reducing their aggregation within mitochondria. Under oxidative stress, both Vms1 and ANKZF1 localize to mitochondria and recruit Cdc48/VCP to facilitate mitochondria‑associated degradation (MAD), although the physiological requirement for this mitochondrial targeting remains unclear. Notably, recent studies in yeast reveal that VMS1 deletion aggravates proteotoxicity in the mitochondrial matrix, highlighting its essential protective role during mitochondrial stress.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for ANKZF1 antibody 20447-1-AP | Download protocol |
| WB protocol for ANKZF1 antibody 20447-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Stalled translation by mitochondrial stress upregulates a CNOT4-ZNF598 ribosomal quality control pathway important for tissue homeostasis | ||
Acta Neuropathol Commun Inefficient quality control of ribosome stalling during APP synthesis generates CAT-tailed species that precipitate hallmarks of Alzheimer's disease | ||
bioRxiv Ribosomal quality control factors inhibit repeat-associated non-AUG translation from GC-rich repeats | ||
Nucleic Acids Res Ribosomal quality control factors inhibit repeat-associated non-AUG translation from GC-rich repeats | ||
Oncol Rep Overexpression of ZNF169 promotes the growth and proliferation of colorectal cancer cells via the upregulation of ANKZF1 | ||
EMBO J The coordinated action of UFMylation and the RQC pathways clears arrested polypeptides at the ER.
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