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验证数据展示

  • WB analysis using 24033-1-AP

    WB analysis using 24033-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24033-1-AP (ANKRD57 antibody) at dilution of 1:60000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 24033-1-AP

    IP experiment of HeLa using 24033-1-AP

    IP result of anti-ANKRD57 (IP:24033-1-AP, 3ug; Detection:24033-1-AP 1:1000) with HeLa cells lysate 3000ug.

  • IHC staining of human cerebellum using 24033-1-AP

    IHC staining of human cerebellum using 24033-1-AP

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 24033-1-AP (ANKRD57 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human cerebellum using 24033-1-AP

    IHC staining of human cerebellum using 24033-1-AP

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 24033-1-AP (ANKRD57 Antibody) at dilution of 1:50 (under 40x lens).

ANKRD57 Polyclonal antibody

ANKRD57 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 24033-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IP, IHC, ELISA

别名

ANKRD57, ankyrin repeat domain 57, C2orf26

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells, HeLa cells, HepG2 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman cerebellum tissue, human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:20000-1:100000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

24033-1-AP targets ANKRD57 in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IP, IHC, ELISA Application Description
文献引用应用IHC
经测试反应性 human
文献引用反应性human
免疫原 ANKRD57 fusion protein Ag21238 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ankyrin repeat domain 57
别名 ANKRD57, ankyrin repeat domain 57, C2orf26
计算分子量 525 aa, 56 kDa
观测分子量 65-70 kDa
GenBank蛋白编号BC036652
基因名称 ANKRD57
Gene ID (NCBI) 65124
RRIDAB_2879412
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen Affinity purified
UNIPROT IDQ53LP3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ANKRD57 antibody 24033-1-APDownload protocol
IHC protocol for ANKRD57 antibody 24033-1-APDownload protocol
IP protocol for ANKRD57 antibody 24033-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Front Immunol

A DNA-Methylation-Driven Genes Based Prognostic Signature Reveals Immune Microenvironment in Pancreatic Cancer.

Authors - Mingjia Xiao
View Article