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验证数据展示

  • WB analysis using 24033-1-AP

    WB analysis using 24033-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24033-1-AP (ANKRD57 antibody) at dilution of 1:60000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 24033-1-AP

    IP experiment of HeLa using 24033-1-AP

    IP result of anti-ANKRD57 (IP:24033-1-AP, 3ug; Detection:24033-1-AP 1:1000) with HeLa cells lysate 3000ug.

  • IHC staining of human cerebellum using 24033-1-AP

    IHC staining of human cerebellum using 24033-1-AP

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 24033-1-AP (ANKRD57 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human cerebellum using 24033-1-AP

    IHC staining of human cerebellum using 24033-1-AP

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 24033-1-AP (ANKRD57 Antibody) at dilution of 1:50 (under 40x lens).

ANKRD57 Polyclonal antibody

ANKRD57 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 24033-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IP, IHC, ELISA

别名

ANKRD57, ankyrin repeat domain 57, C2orf26

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA431 cells, HeLa cells, HepG2 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman cerebellum tissue, human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:20000-1:100000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

24033-1-AP targets ANKRD57 in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IP, IHC, ELISA Application Description
文献引用应用IHC
经测试反应性 human
文献引用反应性human
免疫原

CatNo: Ag21238

Product name: Recombinant human ANKRD57 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-306 aa of BC036652

Sequence: MEGPAEWGPEAALGPEAVLRFLAERGGRALHAELVQHFRGALGGEPEQRARARAHFKELVNAVATVRVDPADGAKYVHLKKRFCEGPSEPSGDPPRIQVTAEPEAPDGPAGPEARDRLPDAAAPESLPGQGRELGEGEPPAPAHWPPLSAGARRKNSRRDVQPLPRTPAPGPSEDLELPPHGCEEADRGSSLVGATAQRPARQNLRDLVMGSSPQLKRSVCPGGSSPGSSSGGGRGRGGGDSDSASVASSSAEEESSGGGSVTLDPLEHAWMLSASDGKWDSLEGLLTCEPGLLVKRDFITGFTCL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ankyrin repeat domain 57
别名 ANKRD57, ankyrin repeat domain 57, C2orf26
计算分子量 525 aa, 56 kDa
观测分子量 65-70 kDa
GenBank蛋白编号BC036652
基因名称 ANKRD57
Gene ID (NCBI) 65124
RRIDAB_2879412
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen Affinity purified
UNIPROT IDQ53LP3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ANKRD57 antibody 24033-1-APDownload protocol
IHC protocol for ANKRD57 antibody 24033-1-APDownload protocol
IP protocol for ANKRD57 antibody 24033-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Front Immunol

A DNA-Methylation-Driven Genes Based Prognostic Signature Reveals Immune Microenvironment in Pancreatic Cancer.

Authors - Mingjia Xiao
View Article