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验证数据展示

  • WB analysis of HeLa using 24203-1-AP

    WB analysis of HeLa using 24203-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 24203-1-AP (ANKRD55 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human cervical cancer using 24203-1-AP

    IHC staining of human cervical cancer using 24203-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 24203-1-AP (ANKRD55 Antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HeLa using 24203-1-AP

    IF Staining of HeLa using 24203-1-AP

    Immunofluorescent analysis of HeLa cells using 24203-1-AP (ANKRD55 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

ANKRD55 Polyclonal antibody

ANKRD55 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 24203-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

ANKRD55, ankyrin repeat domain 55

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, L02 cells
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24203-1-AP targets ANKRD55 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 ANKRD55 fusion protein Ag21271 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ankyrin repeat domain 55
别名 ANKRD55, ankyrin repeat domain 55
计算分子量 614 aa, 68 kDa
观测分子量 68 kDa
GenBank蛋白编号BC106914
基因名称 ANKRD55
Gene ID (NCBI) 79722
RRIDAB_2879456
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ3KP44
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ANKRD55 also named as Ankyrin repeat domain containing protein 55 is a 614 amino acid protein, which contains 9 ANK repeat. ANKRD55 may exist three alternatively spliced isoforms and belongs to ankyrin family. The function of ANKRD55 is still non-known and need to be further studied.

实验方案

Product Specific Protocols
WB protocol for ANKRD55 antibody 24203-1-APDownload protocol
IHC protocol for ANKRD55 antibody 24203-1-APDownload protocol
IF protocol for ANKRD55 antibody 24203-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols