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验证数据展示

  • WB analysis using 24203-1-AP

    WB analysis using 24203-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24203-1-AP (ANKRD55 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 24203-1-AP

    WB analysis of HeLa using 24203-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 24203-1-AP (ANKRD55 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human cervical cancer using 24203-1-AP

    IHC staining of human cervical cancer using 24203-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 24203-1-AP (ANKRD55 Antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HeLa using 24203-1-AP

    IF Staining of HeLa using 24203-1-AP

    Immunofluorescent analysis of HeLa cells using 24203-1-AP (ANKRD55 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

ANKRD55 Polyclonal antibody

ANKRD55 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 24203-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

Ankyrin repeat domain-containing protein 55

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, L02 cells, mouse spleen tissue, mouse small intestine tissue, mouse thymus tissue
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24203-1-AP targets ANKRD55 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag21271

Product name: Recombinant human ANKRD55 protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 1-326 aa of BC106914

Sequence: MDSNLRDINESTPLAYALYCGHTACVKLLSQESRTEPTRPPPSQSSRPQKKERRFNVLNQIFCKNKKEEQRAHQKDPSRDRYREEDTSEVNDIITTFDSIVGTNCQEQPGDQVAMVEFKKKTSDNSKYLLPEKKPLARKGLPPIRTQSLPPITLGNNFLTASHRATSHAGLSSAPHHMAQRSQKSRSEQDLLNNRTGCQMLLDNPWKSDSNQVFSYKVWTVSSSDKLLDRLLSVRPGHQEVSVPPHLRHLHNPSSGQNFQHLSPNRHKIRDLPFTRNNLAPLPDQKFLSGEPLRTNRVLPAIPSQRRHSTAAEESEHSANPTSDEN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ankyrin repeat domain 55
别名 Ankyrin repeat domain-containing protein 55
计算分子量 614 aa, 68 kDa
观测分子量 68 kDa
GenBank蛋白编号BC106914
基因名称 ANKRD55
Gene ID (NCBI) 79722
RRIDAB_2879456
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ3KP44
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ANKRD55 also named as Ankyrin repeat domain containing protein 55 is a 614 amino acid protein, which contains 9 ANK repeat. ANKRD55 may exist three alternatively spliced isoforms and belongs to ankyrin family. The function of ANKRD55 is still non-known and need to be further studied. ANKRD55 is a critical regulator of TCR signaling and a key regulator of T cell inflammation in multiple sclerosis (PMID: 41090353).

实验方案

Product Specific Protocols
IF protocol for ANKRD55 antibody 24203-1-APDownload protocol
IHC protocol for ANKRD55 antibody 24203-1-APDownload protocol
WB protocol for ANKRD55 antibody 24203-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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