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验证数据展示

  • WB analysis using 27568-1-AP

    WB analysis using 27568-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27568-1-AP (ANKRD46 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • IF Staining of HEK-293 using 27568-1-AP

    IF Staining of HEK-293 using 27568-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using ANKRD46 antibody (27568-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

ANKRD46 Polyclonal antibody

ANKRD46 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 27568-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

Ankyrin repeat domain-containing protein 46, ankyrin repeat domain 46, ANK-S

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IF/ICC detected inHEK-293 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:5000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27568-1-AP targets ANKRD46 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 ANKRD46 fusion protein Ag26623 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ankyrin repeat domain 46
别名 Ankyrin repeat domain-containing protein 46, ankyrin repeat domain 46, ANK-S
观测分子量 25 kDa
GenBank蛋白编号BC060843
基因名称 ANKRD46
Gene ID (NCBI) 157567
RRIDAB_3669610
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ86W74
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ANKRD46 encodes a protein containing multiple ankyrin repeats. Ankyrin domains function in protein-protein interactions in a variety of cellular processes. ANKRD46 also known as ankyrin repeat small protein (ANK-S), is a 228-amino acid single-pass membrane protein(PMID: 21219636).

实验方案

Product Specific Protocols
WB protocol for ANKRD46 antibody 27568-1-APDownload protocol
IF protocol for ANKRD46 antibody 27568-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols