验证数据展示
经过测试的应用
| Positive IP detected in | MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
25765-1-AP targets ANKRD40 in IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | IP, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag22731 Product name: Recombinant human ANKRD40 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-111 aa of BC005853 Sequence: MQELVLKVRIQNPSLRENDFIEIELDRQELTYQELLRVCCCELGVNPDQVEKIRKLPNTLLRKDKDVARLQDFQELELVLMISENNFLFRNAASTLTERPCYNRRASKLTY 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ankyrin repeat domain 40 |
| 别名 | ankyrin repeat domain 40 |
| 计算分子量 | 368 aa, 41 kDa |
| 观测分子量 | 41 kDa |
| GenBank蛋白编号 | BC005853 |
| 基因名称 | ANKRD40 |
| Gene ID (NCBI) | 91369 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q6AI12 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ANKRD40 (ankyrin repeat domain 40) is a protein-coding gene conserved across mammals, including humans, mice, rats, and whales. The encoded protein contains ankyrin repeat domains, which are structural motifs typically involved in protein-protein interactions. In humans, ANKRD40 is located on chromosome 17 (17q21.33) and is ubiquitously expressed, with higher levels in tissues like the brain and adipose. ANKRD40 implicated in DNA replication, cell adhesion and migration; its expression correlates with tumor progression, yet precise molecular mechanisms remain undefined.
实验方案
| Product Specific Protocols | |
|---|---|
| IP protocol for ANKRD40 antibody 25765-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
