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验证数据展示

  • WB analysis of rat testis using 29891-1-AP

    WB analysis of rat testis using 29891-1-AP

    rat testis tissue were subjected to SDS PAGE followed by western blot with 29891-1-AP (ANKRD31 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis using 29891-1-AP

    WB analysis using 29891-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29891-1-AP (ANKRD31 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

ANKRD31 Polyclonal antibody

ANKRD31 Polyclonal Antibody for WB, ELISA
Cat No. 29891-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Rat, mouse, human

应用

WB, ELISA

别名

ANKRD31, ankyrin repeat domain 31, FLJ40191

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inrat testis tissue, mouse heart tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29891-1-AP targets ANKRD31 in WB, ELISA applications and shows reactivity with Rat, mouse, human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Rat, mouse, human
免疫原

CatNo: Ag31858

Product name: Recombinant human ANKRD31 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-200 aa of NM_001164443.1

Sequence: MEEGVQAPDWDSDETVIEGSVTESDLEEKELPWRRLLFDQDASLKSEFSLHPDTRGMCKGMPSPEIQLGFKLREDLQEQMNKNKMMPVLSEDTILQSQDETERNQALLQTRKNCSMFIGSFRQSGLSLNHQNIEGPEAESPEVLPHIEKELSEGRDSPEVSLLSGTAITVSDTVAVKETSLVEPEKILAAPNTFFEPRKE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ankyrin repeat domain 31
别名 ANKRD31, ankyrin repeat domain 31, FLJ40191
计算分子量211 kDa
观测分子量210 kDa
GenBank蛋白编号NM_001164443.1
基因名称 ANKRD31
Gene ID (NCBI) 256006
RRIDAB_3086181
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8N7Z5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ANKRD31 (Ankyrin repeat domain-containing protein 31) controls the number, timing, and location of meiotic DNA breaks during meiosis. It encodes a 1857 amino acid protein with 2 ankyrin repeat domains (PubMed: 31003867). ANKRD31 was a meiotic protein colocalized REC114 on chromatin (PubMed: 31000436). Mutations in ANKRD31 are associated with a Rett syndrome (RTT)-like phenotype (PMID: 27541642). And ANKRD31 has a high expression in testis.

实验方案

Product Specific Protocols
WB protocol for ANKRD31 antibody 29891-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols