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  • Featured Product
  • KD/KO Validated

ANKRD27 Polyclonal antibody

ANKRD27 Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 24034-1-AP

Print datasheet

Synonyms

ANKRD27, PP12899, VARP, VPS9 domain containing protein

验证数据展示

  • WB analysis of MCF-7 using 24034-1-AP

    WB analysis of MCF-7 using 24034-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 24034-1-AP (ANKRD27 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of A431 using 24034-1-AP

    IP experiment of A431 using 24034-1-AP

    IP result of anti-ANKRD27 (IP:24034-1-AP, 4ug; Detection:24034-1-AP 1:1000) with A431 cells lysate 2000ug.

  • IHC staining of human breast cancer using 24034-1-AP

    IHC staining of human breast cancer using 24034-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24034-1-AP (ANKRD27 Antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of MCF-7 using 24034-1-AP

    IF Staining of MCF-7 using 24034-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed MCF-7 cells using 24034-1-AP (ANKRD27 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inMCF-7 cells
Positive IP detected inA431 cells, MCF-7 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 24034-1-AP is ANKRD27 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, IHC, IP, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen ANKRD27 fusion protein Ag21239 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ankyrin repeat domain 27 (VPS9 domain)
Synonyms ANKRD27, PP12899, VARP, VPS9 domain containing protein
Calculated Molecular Weight 1050 aa, 117 kDa
Observed Molecular Weight 117-130 kDa
GenBank Accession NumberBC050529
Gene Symbol ANKRD27
Gene ID (NCBI) 84079
RRIDAB_2879413
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96NW4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

VARP (VPS9-ankyrin-repeat protein, also known as ANKRD27) was originally identified as an N-terminal VPS9 (vacuolar protein sorting 9)-domain-containing protein that possesses guanine nucleotide exchange factor (GEF) activity toward small GTPase Rab21 and contains two ankyrin repeat (ANKR) domains in its central region.

实验方案

Product Specific Protocols
WB protocol for ANKRD27 antibody 24034-1-APDownload protocol
IHC protocol for ANKRD27 antibody 24034-1-APDownload protocol
IF protocol for ANKRD27 antibody 24034-1-APDownload protocol
IP protocol for ANKRD27 antibody 24034-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

EMBO J

Control of RAB7 activity and localization through the retromer-TBC1D5 complex enables RAB7-dependent mitophagy.

Authors - Ana Jimenez-Orgaz
  • KO Validated
View Article
WB

J Cell Sci

TI-VAMP/VAMP7-SNARE-Rab-GTPase interaction network within a ciliary membrane targeting complex.

Authors - Vasundhara Kandachar
View Article
humanWB

Autophagy

RAB21 controls autophagy and cellular energy homeostasis by regulating retromer-mediated recycling of SLC2A1/GLUT1

Authors - Yifei Pei
View Article