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验证数据展示

  • IHC staining of human stomach using 24035-1-AP

    IHC staining of human stomach using 24035-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 24035-1-AP (ANKRD22 antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human stomach using 24035-1-AP

    IHC staining of human stomach using 24035-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 24035-1-AP (ANKRD22 antibody) at dilution of 1:200 (under 40x lens).

  • IF Staining of SW480 using 24035-1-AP

    IF Staining of SW480 using 24035-1-AP

    Immunofluorescent analysis of (4% PFA) fixed SW480 cells using ANKRD22 antibody (24035-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

ANKRD22 Polyclonal antibody

ANKRD22 Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 24035-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, IF/ICC, ELISA

别名

ANKRD2, Ankyrin repeat domain-containing protein 22, ankyrin repeat domain 22

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSW480 cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24035-1-AP targets ANKRD22 in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag21248

Product name: Recombinant human ANKRD22 protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 1-191 aa of BC021671

Sequence: MGILYSEPICQAAYQNDFGQVWRWVKEDSSYANVQDGFNGDTPLICACRRGHVRIVSFLLRRNANVNLKNQKERTCLHHAVKKKFTFIDYLLIILLMPVLLIGYFLMVSKTKQNEALVRMLLDAGVEVNATDCYGCTALHYACEMKNQSLIPLLLEARADPTIKNKHGESSLDIARRLKFSQIELMLRKAL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ankyrin repeat domain 22
别名 ANKRD2, Ankyrin repeat domain-containing protein 22, ankyrin repeat domain 22
计算分子量 191 aa, 22 kDa
GenBank蛋白编号BC021671
基因名称 ANKRD22
Gene ID (NCBI) 118932
RRIDAB_3669429
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen Affinity purified
UNIPROT IDQ5VYY1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ankyrin repeat domain-containing protein 22 (ANKRD22) is a novel N-myristoylated protein with a predicted single-pass transmembrane domain. ANKRD22 could potentially act as a novel therapeutic target for non-small cell lung cancer (NSCLC) (PMID: 28667340).

实验方案

Product Specific Protocols
IF protocol for ANKRD22 antibody 24035-1-APDownload protocol
IHC protocol for ANKRD22 antibody 24035-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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