验证数据展示
经过测试的应用
| Positive IHC detected in | mouse liver tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
24087-1-AP targets ANKRD18A in IHC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | IHC, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 |
CatNo: Ag21335 Product name: Recombinant human ANKRD18A protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 262-390 aa of BC152434 Sequence: NHLRNDNQETAAMKPANLKKRKERAKAEHNLKVASEEKQERLQRSENKQPQDSQSYGKKKDAMYGNFMLKKDIAMLKEELYAIKNDSLRKEKKYIQEIKSITEINANFEKSVRLNEKMITKTVARYSQQ 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ankyrin repeat domain 18A |
| 别名 | ankyrin repeat domain 18A, KIAA2015 |
| 计算分子量 | 992 aa, 116 kDa |
| GenBank蛋白编号 | BC152434 |
| 基因名称 | ANKRD18A |
| Gene ID (NCBI) | 253650 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen Affinity purified |
| UNIPROT ID | Q8IVF6 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ANKRD18A is an ankyrin repeat-containing protein that likely operates in the realm of epigenetic regulation, potentially influencing gene expression via DNA methylation mechanisms. Although its exact biochemical roles remain unclear, its frequent promoter hypermethylation in lung cancer, along with germline variation in vulvar skin disorders, underscores a compelling link to disease pathogenesis. Located on chromosome 9p13.1, ANKRD18A remains a compelling subject for further functional and clinical exploration.
