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验证数据展示

  • WB analysis using 27701-1-AP

    WB analysis using 27701-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27701-1-AP (ANKRD17 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 27701-1-AP

    IHC staining of human ovary cancer using 27701-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 27701-1-AP (ANKRD17 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ANKRD17 antibody included

ANKRD17 Polyclonal antibody

ANKRD17 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27701-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

NY BR 16, KIAA0697, GTAR, ankyrin repeat domain 17

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293T cells, HeLa cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy ANKRD17 Ready-To-Use IHC Kit. ANKRD17 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27701-1-AP targets ANKRD17 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 ANKRD17 fusion protein Ag26767 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ankyrin repeat domain 17
别名 NY BR 16, KIAA0697, GTAR, ankyrin repeat domain 17
计算分子量 274 kDa
观测分子量270-300 kDa
GenBank蛋白编号NM_001286771
基因名称 ANKRD17
Gene ID (NCBI) 26057
RRIDAB_3085987
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO75179
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ANKRD17 belongs to the family of ankyrin repeat-containing proteins, and contains two distinct arrays of ankyrin repeats in its amino-terminal region. It also contains a nuclear export signal, nuclear localization signal, and a cyclin-binding RXL motif. Localization of this protein to the nucleus has been shown experimentally, and interactions between this protein and cyclin-dependent kinase 2 have been observed.

实验方案

Product Specific Protocols
WB protocol for ANKRD17 antibody 27701-1-APDownload protocol
IHC protocol for ANKRD17 antibody 27701-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols