验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, mouse liver tissue, PC-3 cells |
Positive IHC detected in | human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
24334-1-AP targets ANKRD16 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
经测试反应性 | human, mouse |
免疫原 | ANKRD16 fusion protein Ag19369 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | ankyrin repeat domain 16 |
别名 | Ankyrin repeat domain-containing protein 16, ankyrin repeat domain 16 |
计算分子量 | 361 aa, 39 kDa |
观测分子量 | 39-45 kDa |
GenBank蛋白编号 | BC062346 |
基因名称 | ANKRD16 |
Gene ID (NCBI) | 54522 |
RRID | AB_2879499 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q6P6B7 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ANKRD16, a vertebrate-specific protein that contains ankyrin repeats, binds directly to the catalytic domain of AlaRS. Serine that is misactivated by AlaRS is captured by the lysine side chains of ANKRD16, which prevents the charging of serine adenylates to tRNAAla and precludes serine misincorporation in nascent peptides. The deletion of Ankrd16 in the brains of Aarssti/sti mice causes widespread protein aggregation and neuron loss (PMID: 29769718).
实验方案
Product Specific Protocols | |
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WB protocol for ANKRD16 antibody 24334-1-AP | Download protocol |
IHC protocol for ANKRD16 antibody 24334-1-AP | Download protocol |
IF protocol for ANKRD16 antibody 24334-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |