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ANKRD13A Polyclonal antibody

ANKRD13A Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat No : 23998-1-AP

Print datasheet

Synonyms

ANKRD13, ANKRD13A, ankyrin repeat domain 13A, NY REN 25, Protein KE03

验证数据展示

  • WB analysis using 23998-1-AP

    WB analysis using 23998-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23998-1-AP (ANKRD13A antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells, HepG2 cells, LNCaP cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23998-1-AP targets ANKRD13A in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA
Tested Reactivity human
Immunogen ANKRD13A fusion protein Ag21200 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ankyrin repeat domain 13A
Synonyms ANKRD13, ANKRD13A, ankyrin repeat domain 13A, NY REN 25, Protein KE03
Calculated Molecular Weight 590 aa, 68 kDa
Observed Molecular Weight 67 kDa
GenBank Accession NumberBC032833
Gene Symbol ANKRD13A
Gene ID (NCBI) 88455
RRIDAB_2879397
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ8IZ07
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ANKRD13A, also named as ANKRD13, a 590 amino acid protein, which contains two ANK repeats and four UIM (ubiquitin-interacting motif) repeats. ANKRD13A localizes in the cell membrane. ANKRD13A as ubiquitin-binding protein that specifically recognizes and binds 'Lys-63'-linked ubiquitin, not bind 'Lys-48'-linked ubiquitin. ANKRD13A positively regulates the internalization of ligand-activated EGFR by binding to the Ub moiety of ubiquitinated EGFR at the cell membrane.

实验方案

Product Specific Protocols
WB protocol for ANKRD13A antibody 23998-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols