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ANKLE1 Polyclonal antibody

ANKLE1 Polyclonal Antibody for IF/ICC, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 24080-1-AP

Print datasheet

Synonyms

ANKLE1, ANKRD41, LEM3, LEMD6

验证数据展示

  • WB analysis of HeLa using 24080-1-AP

    WB analysis of HeLa using 24080-1-AP

    Hela cells were subjected to SDS PAGE followed by western blot with 24080-1-AP (ANKLE1 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 24080-1-AP

    IHC staining of human breast cancer using 24080-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24080-1-AP (ANKLE1 Antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HeLa using 24080-1-AP

    IF Staining of HeLa using 24080-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 24080-1-AP (ANKLE1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHela cells, K-562 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 24080-1-AP is ANKLE1 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, IHC, WB,ELISA
Cited ApplicationsWB, IHC
Tested Reactivity human
Cited Reactivityhuman
Immunogen ANKLE1 fusion protein Ag21253 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ankyrin repeat and LEM domain containing 1
Synonyms ANKLE1, ANKRD41, LEM3, LEMD6
Calculated Molecular Weight 615 aa, 67 kDa
Observed Molecular Weight 67 kDa
GenBank Accession NumberBC125274
Gene Symbol ANKLE1
Gene ID (NCBI) 126549
RRIDAB_2879428
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ8NAG6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ANKLE1 antibody 24080-1-APDownload protocol
IHC protocol for ANKLE1 antibody 24080-1-APDownload protocol
IF protocol for ANKLE1 antibody 24080-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

World J Surg Oncol

Collective analysis of the expression and prognosis for LEM-domain proteins in prostate cancer.

Authors - Tianzhen He
View Article