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验证数据展示

  • WB analysis using 31080-1-AP (same clone as 31080-1-PBS)

    WB analysis using 31080-1-AP (same clone as 31080-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31080-1-AP (ANK2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31080-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse cerebellum using 31080-1-AP (same clone as 31080-1-PBS)

    IHC staining of mouse cerebellum using 31080-1-AP (same clone as 31080-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 31080-1-AP (ANK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31080-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 31080-1-AP (same clone as 31080-1-PBS)

    IHC staining of mouse heart using 31080-1-AP (same clone as 31080-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 31080-1-AP (ANK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31080-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse heart using 31080-1-AP (same clone as 31080-1-PBS)

    IF Staining of mouse heart using 31080-1-AP (same clone as 31080-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using ANK2 antibody (31080-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31080-1-PBS in a different storage buffer formulation.

ANK2 Polyclonal antibody, PBS Only

ANK2 Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 31080-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, Indirect ELISA

别名

ANK 2, Ankyrin 2

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

31080-1-PBS targets ANK2 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag34642

Product name: Recombinant human ANK2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 3571-3708 aa of NM_001148

Sequence: ERLAYIADHLGFSWTELARELDFTEEQIHQIRIENPNSLQDQSHALLKYWLERDGKHATDTNLVECLTKINRMDIVHLMETNTEPLQERISHSYAEIEQTITLDHSEGFSVLQEELCTAQHKQKEEQAVSKESETCDH

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ankyrin 2, neuronal
别名 ANK 2, Ankyrin 2
计算分子量434 kDa
观测分子量250-260 kDa
GenBank蛋白编号NM_001148
基因名称 ANK2
Gene ID (NCBI) 287
RRIDAB_3669845
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ01484
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The Ankyrins, belonging to the adapter protein family, consist of Ankyrin-R (ANK1), Ankyrin-B (ANK2), and Ankyrin-G (ANK3). Initially, ANK2 was considered as a key cytoskeletal-associated protein in the brain. Besides, it was discovered as a pivotal adaptor and scaffolding protein in the heart. Remarkably, recent studies have demonstrated that Ankyrins contribute to the modulation of various biological activities in cancers. (PMID: 36539782)

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