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验证数据展示

  • WB analysis of HEK-293 using 31497-1-AP

    WB analysis of HEK-293 using 31497-1-AP

    WB result of ANGPTL4 antibody (31497-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-ANGPTL4 transfected HEK-293 cells.

  • WB analysis using 31497-1-AP

    WB analysis using 31497-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31497-1-AP (ANGPTL4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 31497-1-AP

    IHC staining of human placenta using 31497-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 31497-1-AP (ANGPTL4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HEK-293 using 31497-1-AP

    IF Staining of HEK-293 using 31497-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using ANGPTL4 antibody (31497-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IF Staining of NIH/3T3 using 31497-1-AP

    IF Staining of NIH/3T3 using 31497-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using ANGPTL4 antibody (31497-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IHC staining of human placenta using 31497-1-AP

    IHC staining of human placenta using 31497-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 31497-1-AP (ANGPTL4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HT-29 using 31497-1-AP

    IF Staining of HT-29 using 31497-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HT-29 cells using ANGPTL4 antibody (31497-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

ANGPTL4 Polyclonal antibody

ANGPTL4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 31497-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

angiopoietin like 4, Angiopoietin like protein 4, Angiopoietin related protein 4, Angiopoietin-like protein 4, Angiopoietin-related protein 4

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse heart tissue, HEK-293 cells, rat heart tissue
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells, NIH/3T3 cells, HT-29 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31497-1-AP targets ANGPTL4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag31212

Product name: Recombinant human ANGPTL4 protein

Source: e coli.-derived, PKG

Tag: GST

Domain: 26-200 aa of BC023647

Sequence: GPVQSKSPRFASWDEMNVLAHGLLQLGQGLREHAERTRSQLSALERRLSACGSACQGTEGSTDLPLAPESRVDPEVLHSLQTQLKAQNSRIQQLFHKVAQQQRHLEKQHLRIQHLQSQFGLLDHKHLDHEVAKPARRKRLPEMAQPVDPAHNVSRLHRLPRDCQELFQVGERQSG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 angiopoietin-like 4
别名 angiopoietin like 4, Angiopoietin like protein 4, Angiopoietin related protein 4, Angiopoietin-like protein 4, Angiopoietin-related protein 4
计算分子量 45 kDa
观测分子量 60 kDa
GenBank蛋白编号BC023647
基因名称 ANGPTL4
Gene ID (NCBI) 51129
RRIDAB_3742439
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ9BY76
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Angiopoietin-like protein 4 (ANGPTL4), a member of the angiopoietin-like protein subfamily, is a secretory protein that functions as an important modulator of glucose and lipid metabolism. ANGPTL4 inhibits lipoprotein lipase (LPL)-dependent lipolysis thereby limiting the uptake of free fatty acids. Overexpression of ANGPTL4 results in hypertriglyceridemia, while ANGPTL4 deficiency suppresses foam formation in macrophages and protects against atherosclerosis. ANGPTL4 may play a role in several cancers and it also has been shown to prevent the metastatic process by inhibiting vascular activity as well as tumor cell motility and invasiveness. Decreased expression of this protein has been associated with type 2 diabetes.

实验方案

Product Specific Protocols
IF protocol for ANGPTL4 antibody 31497-1-APDownload protocol
IHC protocol for ANGPTL4 antibody 31497-1-APDownload protocol
WB protocol for ANGPTL4 antibody 31497-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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