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验证数据展示

  • WB analysis using 67348-1-Ig (same clone as 67348-1-PBS)

    WB analysis using 67348-1-Ig (same clone as 67348-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67348-1-Ig (ANAPC5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67348-1-PBS in a different storage buffer formulation.

  • IF Staining of human breast cancer using 67348-1-Ig (same clone as 67348-1-PBS)

    IF Staining of human breast cancer using 67348-1-Ig (same clone as 67348-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using ANAPC5 antibody (67348-1-Ig, Clone: 2A7G5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67348-1-PBS in a different storage buffer formulation.

  • Cytometric bead array sample test of MP51600-1

    Cytometric bead array sample test of MP51600-1

    Cytometric bead array sample test of MP51600-1, ANAPC5 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67348-1-PBS. Detection antibody: 67348-2-PBS.

  • IHC staining of human breast cancer using 67348-1-Ig (same clone as 67348-1-PBS)

    IHC staining of human breast cancer using 67348-1-Ig (same clone as 67348-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67348-1-Ig (ANAPC5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67348-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ANAPC5 antibody included
  • IHC staining of human breast cancer using 67348-1-Ig (same clone as 67348-1-PBS)

    IHC staining of human breast cancer using 67348-1-Ig (same clone as 67348-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67348-1-Ig (ANAPC5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67348-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ANAPC5 antibody included
  • Cytometric bead array standard curve of MP51600-1

    Cytometric bead array standard curve of MP51600-1

    Cytometric bead array standard curve of MP51600-1, ANAPC5 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67348-1-PBS. Detection antibody: 67348-2-PBS. Standard:Ag7409. Range: 6.25-400 ng/mL.

ANAPC5 Monoclonal antibody, PBS Only (Capture)

ANAPC5 Monoclonal Antibody for WB, IHC, IF-P, Cytometric bead array, Indirect ELISA
Cat No. 67348-1-PBS

产品说明书

CloneNo. 2A7G5

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

别名

2A7G5, Anaphase-promoting complex subunit 5, APC5, Cyclosome subunit 5

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy® ANAPC5 Ready-To-Use IHC Kit. ANAPC5 primary antibody included.

产品信息

67348-1-PBS targets ANAPC5 as part of a matched antibody pair:

MP51600-1: 67348-1-PBS capture and 67348-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF-P, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag7409

Product name: Recombinant human ANAPC5 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-343 aa of BC001081

Sequence: MASVHESLYFNPMMTNGVVHANVFGIKDWVTPYKIAVLVLLNEMSRTGEGAVSLMERRRLNQLLLPLLQGPDITLSKLYKLIEESCPQLANSVQIRIKLMAEGELKDMEQFFDDLSDSFSGTEPEVHKTSVVGLFLRHMILAYSKLSFSQVFKLYTALQQYFQNGEKKTVEDADMELTSRDEGERKMEKEELDVSVREEEVSCSGPLSQKQAEFFLSQQASLLKNDETKALTPASLQKELNNLLKFNPDFAEAHYLSYLNNLRVQDVFSSTHSLLHYFDRLILTGAESKSNGEEGYGRSLRYAALNLAALHCRFGHYQQAELALQEAIRIAQESNDHVCLQHC

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 anaphase promoting complex subunit 5
别名 2A7G5, Anaphase-promoting complex subunit 5, APC5, Cyclosome subunit 5
计算分子量 85 kDa
观测分子量 85 kDa, 72 kDa
GenBank蛋白编号BC001081
基因名称 ANAPC5
Gene ID (NCBI) 51433
RRIDAB_2882606
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ9UJX4
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C
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