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APC4 Polyclonal antibody

APC4 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 14129-1-AP

Print datasheet

Synonyms

ANAPC4, APC4, Cyclosome subunit 4

验证数据展示

  • WB analysis using 14129-1-AP

    WB analysis using 14129-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14129-1-AP (APC4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 14129-1-AP

    IHC staining of mouse brain using 14129-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14129-1-AP (APC4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 14129-1-AP

    IHC staining of mouse brain using 14129-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14129-1-AP (APC4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHEK-293 cells, human brain tissue, Hela cells, MCF-7 cells, HIN/3T3 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14129-1-AP targets APC4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen APC4 fusion protein Ag5225 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name anaphase promoting complex subunit 4
Synonyms ANAPC4, APC4, Cyclosome subunit 4
Calculated Molecular Weight 92 kDa
Observed Molecular Weight 92 kDa, 63 kDa
GenBank Accession NumberBC059383
Gene Symbol ANAPC4
Gene ID (NCBI) 29945
RRIDAB_10640671
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UJX5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for APC4 antibody 14129-1-APDownload protocol
IHC protocol for APC4 antibody 14129-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols