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验证数据展示

  • IF Staining of HeLa using 83570-4-RR

    IF Staining of HeLa using 83570-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ANAPC10 antibody (83570-4-RR, Clone: 240492E7 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 83570-4-RR

    Affinity and Kinetic Characterization of 83570-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83570-4-RR against Human ANAPC10 were performed. The affinity constant is 0.141 nM.

ANAPC10 Recombinant monoclonal antibody

ANAPC10 Uni-rAb® Recombinant Antibody for IF/ICC, ELISA
Cat No. 83570-4-RR

产品说明书

CloneNo. 240492E7
引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

IF/ICC, ELISA and More (1)

别名

DOC1, APC10, Anaphase-promoting complex subunit 10, ANAPC 10, 240492E7

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy® ANAPC10 Ready-To-Use IHC Kit. ANAPC10 primary antibody included.

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

83570-4-RR targets ANAPC10 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human, mouse
免疫原

CatNo: Ag8487

Product name: Recombinant human ANAPC10 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-185 aa of BC005217

Sequence: MTTPNKTPPGADPKQLERTGTVREIGSQAVWSLSSCKPGFGVDQLRDDNLETYWQSDGSQPHLVNIQFRRKTTVKTLCIYADYKSDESYTPSKISVRVGNNFHNLQEIRQLELVEPSGWIHVPLTDNHKKPTRTFMIQIAVLANHQNGRDTHMRQIKIYTPVEESSIGKFPRCTTIDFMMYRSIR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 anaphase promoting complex subunit 10
别名 DOC1, APC10, Anaphase-promoting complex subunit 10, ANAPC 10, 240492E7
计算分子量 185 aa, 21 kDa
GenBank蛋白编号BC005217
基因名称 ANAPC10
Gene ID (NCBI) 10393
RRIDAB_3671183
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ9UM13
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The anaphase promoting complex/cyclosome (APC/C) is a cell cycle-regulated multimeric E3 ubiquitin ligase assembled from 13 individual subunits. The anaphase promoting complex subunit 10 (APC10) is a core subunit of APC/C that is highly conserved in humans. APC10 genetically and physically interacts with a series of subunits of the APC/C and is necessary for the ubiquitination activity of APC/C by enhancing the affinity of the APC/C for its substrate.

实验方案

Product Specific Protocols
IF protocol for ANAPC10 antibody 83570-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB

Arch Oral Biol

Anaphase promoting complex subunit 10 is a potential diagnostic and prognostic biomarker in oral squamous cell carcinoma

Authors - Lu Jia
View Article
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