验证数据展示

WB analysis of HeLa using 21748-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 21748-1-AP (APC1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis using 21748-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 21748-1-AP (APC1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis using 21748-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 21748-1-AP (APC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of mouse testis using 21748-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 21748-1-AP (APC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with APC1 antibody included
IHC staining of mouse testis using 21748-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 21748-1-AP (APC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with APC1 antibody included
IHC staining of mouse kidney using 21748-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 21748-1-AP (APC1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with APC1 antibody included
IHC staining of human stomach cancer using 21748-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 21748-1-AP (APC1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with APC1 antibody included
IHC staining of mouse stomach using 21748-1-AP
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 21748-1-AP (APC1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with APC1 antibody included
IHC staining of rat kidney using 21748-1-AP
Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 21748-1-AP (APC1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with APC1 antibody included
IHC staining of rat stomach using 21748-1-AP
Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 21748-1-AP (APC1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with APC1 antibody included

APC1 Polyclonal antibody

APC1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 21748-1-AP

产品说明书

引用文献(8)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA and More (1)

别名

ANAPC1, Anaphase-promoting complex subunit 1, Apc 1, Mitotic checkpoint regulator, Testis-specific gene 24 protein

缓冲液配方: PBS, Azide, Glycerol

PBS, Azide, Glycerol

偶联物: Unconjugated

Unconjugated

规格	价格	库存

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概述

经过测试的应用
产品信息
发表文章 (8)
实验方案

产品说明书

MSDS

经过测试的应用

Positive WB detected in	MCF-7 cells, HeLa cells, K-562 cells, Transfected HEK-293 cells, HT-1080 cells, MDA-MB-231 cells
Positive IHC detected in	mouse testis tissue, human cervical cancer tissue, human stomach tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Planning an IHC experiment? We recommend our IHCeasy APC1 Ready-To-Use IHC Kit. APC1 primary antibody included.

应用	推荐稀释比
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WB	See 8 publications below
IHC	See 1 publications below
IF	See 1 publications below

产品信息

21748-1-AP targets APC1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用	WB, IHC, ELISA Application Description
文献引用应用	WB, IHC, IF
经测试反应性	human, mouse, rat
文献引用反应性	human, mouse
免疫原	CatNo: Ag13399 Product name: Recombinant human ANAPC1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1596-1944 aa of BC104902 Sequence: STDNRYHLQALRHLYVLAAEPRLLVPVDVDTNTPCYALLEVTYKGTQWYEQTKEELMAPTLLPELHLLKQIKVKGPRYWELLIDLSKGTQHLKSILSKDGVLYVKLRAGQLSYKEDPMGWQSLLAQTVANRNSEARAFKPETISAFTSDPALLSFAEYFCKPTVNMGQKQEILDLFSSVLYECVTQETPEMLPAYIAMDQAIRRLGRREMSETSELWQIKLVLEFFSSRSHQERLQNHPKRGLFMNSEFLPVVKCTIDNTLDQWLQVGGDMCVHAYLSGQPLEESQLSMLACFLVYHSVPAPQHLPPIGLEGSTSFAELLFKFKQLKMPVRALLRLAPLLLGNPQPMVM 种属同源性预测
宿主/亚型	Rabbit / IgG
抗体类别	Polyclonal
产品类型	Antibody
全称	anaphase promoting complex subunit 1
别名	ANAPC1, Anaphase-promoting complex subunit 1, Apc 1, Mitotic checkpoint regulator, Testis-specific gene 24 protein
计算分子量	1944 aa, 217 kDa
观测分子量	200-210 kDa
GenBank蛋白编号	BC104902
基因名称	APC1
Gene ID (NCBI)	64682
RRID	AB_10733241
偶联类型	Unconjugated
形式	Liquid
纯化方式	Antigen affinity purification
UNIPROT ID	Q9H1A4
储存缓冲液	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

实验方案

Product Specific Protocols
IHC protocol for APC1 antibody 21748-1-AP	Download protocol
WB protocol for APC1 antibody 21748-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
human	WB	Nat Commun DNA replication initiation factor RECQ4 possesses a role in antagonizing DNA replication initiation Authors - Xiaohua Xu View Article
human	WB	Oncotarget Screening key microRNAs for castration-resistant prostate cancer based on miRNA/mRNA functional synergistic network. Authors - Jin Zhu View Article
human	WB	Sci Rep Reciprocal interaction between SIRT6 and APC/C regulates genomic stability. Authors - Helin Wang View Article
human	WB	Cell Rep TRPS1 drives heterochromatic origin refiring and cancer genome evolution. Authors - Jianguo Yang View Article
human	WB	Cell Biosci PATL2 mutations affect human oocyte maternal mRNA homeostasis and protein interactions in cell cycle regulation Authors - Yin-Li Zhang View Article
human	WB,IHC	Cancer Control The Potential Biological Roles and Clinical Significance of Anaphase-Promoting Complex Subunit 1 in Colorectal Cancer Authors - Yi Chen View Article

验证数据展示

WB analysis of HeLa using 21748-1-AP

WB analysis using 21748-1-AP

WB analysis using 21748-1-AP

IHC staining of mouse testis using 21748-1-AP

IHC staining of mouse testis using 21748-1-AP

IHC staining of mouse kidney using 21748-1-AP

IHC staining of human stomach cancer using 21748-1-AP

IHC staining of mouse stomach using 21748-1-AP

IHC staining of rat kidney using 21748-1-AP

IHC staining of rat stomach using 21748-1-AP

APC1 Polyclonal antibody

APC1 Polyclonal Antibody for WB, IHC, ELISA

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

实验方案

发表文章

DNA replication initiation factor RECQ4 possesses a role in antagonizing DNA replication initiation

Screening key microRNAs for castration-resistant prostate cancer based on miRNA/mRNA functional synergistic network.

Reciprocal interaction between SIRT6 and APC/C regulates genomic stability.

TRPS1 drives heterochromatic origin refiring and cancer genome evolution.

PATL2 mutations affect human oocyte maternal mRNA homeostasis and protein interactions in cell cycle regulation

The Potential Biological Roles and Clinical Significance of Anaphase-Promoting Complex Subunit 1 in Colorectal Cancer

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