Amylase Alpha Monoclonal antibody
Amylase Alpha Monoclonal Antibody for IF/ICC, IHC, WB, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
human, rat, pig
Applications
IF/ICC, IHC, WB, ELISA
Conjugate
Unconjugated
CloneNo.
2F1G8
验证数据展示
经过测试的应用
Positive WB detected in | human saliva, tissue, pig pancreas tissue, rat pancreas tissue |
Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | BxPC-3 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF) | IF : 1:10-1:100 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
IHC | See 2 publications below |
IF | See 1 publications below |
产品信息
The immunogen of 66133-1-Ig is Amylase Alpha Fusion Protein expressed in E. coli.
Tested Applications | IF/ICC, IHC, WB, ELISA |
Cited Applications | IF, IHC |
Tested Reactivity | human, rat, pig |
Cited Reactivity | human, pig |
Immunogen | Amylase Alpha fusion protein Ag8819 种属同源性预测 |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Full Name | amylase, alpha 2A (pancreatic) |
Synonyms | AMY2A, Amylase Alpha, Amylase, alpha, Pancreatic alpha amylase |
Calculated Molecular Weight | 511 aa, 58 kDa |
Observed Molecular Weight | 58 kDa |
GenBank Accession Number | BC007060 |
Gene Symbol | AMY2A |
Gene ID (NCBI) | 279 |
RRID | AB_2881532 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P04746 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for Amylase Alpha antibody 66133-1-Ig | Download protocol |
IHC protocol for Amylase Alpha antibody 66133-1-Ig | Download protocol |
IF protocol for Amylase Alpha antibody 66133-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Am J Physiol Gastrointest Liver Physiol Is the intestinal mucous layer a NAtural Deep Eutectic Solvent (NADES) based digestion matrix | ||
Biochem Biophys Rep Multiple cystic sphere formation from PK-8 cells in three-dimensional culture |