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AMPK Beta 2 Monoclonal antibody

AMPK Beta 2 Monoclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

IF/ICC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

2D5C1

Cat No : 66579-1-Ig

Print datasheet

Synonyms

AMPK subunit beta 2, PRKAB2

验证数据展示

  • WB analysis using 66579-1-Ig

    WB analysis using 66579-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66579-1-Ig (AMPK beta 2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 66579-1-Ig

    IF Staining of HeLa using 66579-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using AMPK Beta 2 antibody (66579-1-Ig, Clone: 2D5C1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inA375 cells, A431 cells, HeLa cells, A549 cells, human brain tissue, rat brain tissue, mouse brain tissue
Positive IF detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:3000
Immunofluorescence (IF)IF : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66579-1-Ig targets AMPK Beta 2 in WB, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications IF/ICC, WB, ELISA
Tested Reactivity Human, mouse, rat
Immunogen AMPK Beta 2 fusion protein Ag5806 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name protein kinase, AMP-activated, beta 2 non-catalytic subunit
Synonyms AMPK subunit beta 2, PRKAB2
Calculated Molecular Weight 30 kDa
Observed Molecular Weight 33 kDa
GenBank Accession NumberBC053610
Gene Symbol PRKAB2
Gene ID (NCBI) 5565
RRIDAB_2881939
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO43741
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AMPK beta 2 is a regulatory subunit of the AMP-activated protein kinase (AMPK), which is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. AMPK beta 2 may be a positive regulator of AMPK activity. It is highly expressed in skeletal muscle and thus may have tissue-specific roles.

实验方案

Product Specific Protocols
WB protocol for AMPK Beta 2 antibody 66579-1-IgDownload protocol
IF protocol for AMPK Beta 2 antibody 66579-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols