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验证数据展示

  • WB analysis using 26907-1-AP

    WB analysis using 26907-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26907-1-AP (AMPK Beta 1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of Jurkat using 26907-1-AP

    WB analysis of Jurkat using 26907-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 26907-1-AP (AMPK beta 1 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

AMPK Beta 1 Polyclonal antibody

AMPK Beta 1 Polyclonal Antibody for WB, ELISA
Cat No. 26907-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA

别名

PRKAB1, AMPKb, AMPK β, AMPK subunit beta-1, AMPK subunit beta 1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA431 cells, Jurkat cells, NIH/3T3 cells, HeLa cells, PC-12 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26907-1-AP targets AMPK Beta 1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag25534

Product name: Recombinant human PRKAB1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-70 aa of BC001056

Sequence: MGNTSSERAALERHGGHKTPRRDSSGGTKDGDRPKILMDSPEDADLFHSEEIKAPEKEEFLAWQHDLEVN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 protein kinase, AMP-activated, beta 1 non-catalytic subunit
别名 PRKAB1, AMPKb, AMPK β, AMPK subunit beta-1, AMPK subunit beta 1
计算分子量 30 kDa
观测分子量 38 kDa
GenBank蛋白编号BC001056
基因名称 PRKAB1
Gene ID (NCBI) 5564
RRIDAB_2880679
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y478
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AMPK Beta 1 (5'-AMP-activated protein kinase subunit beta-1) is also named as PRKAB1 and AMPK. AMPK, a serine/threonine kinase that exists as a heterotrimer comprised of a catalytic α-subunit and regulatory β- and γ-subunits, has been recognized as a sensor of cellular energy homeostasis (PMID: 21937710). AMPK regulates key metabolic enzymes, cell growth, apoptosis, gene transcription, and protein synthesis (PMID: 12829246). AMPK is an energy sensor and plays an essential role in the control of cellular bioenergetics by responding to various stresses including those that induce changes in the cellular AMP:ATP ratio or modulation in intracellular calcium (PMID: 27812976, PMID: 26616193). Recent studies have shown that AMPK mediates the inhibition of cell proliferation and growth of tumor cells (PMID: 16613876). AMPK also inhibits the expression of Glut1 and glycolysis in Tregs by inhibiting mTORC1 signaling (PMID: 25477880).

实验方案

Product Specific Protocols
WB protocol for AMPK Beta 1 antibody 26907-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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