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验证数据展示

  • WB analysis of HEK-293 using 18167-1-AP (same clone as 18167-1-PBS)

    WB analysis of HEK-293 using 18167-1-AP (same clone as 18167-1-PBS)

    WB result of AMPK alpha 2 antibody (18167-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-AMPK alpha 2 transfected HEK-293 cells. This data was developed using the same antibody clone with 18167-1-PBS in a different storage buffer formulation.

  • WB analysis using 18167-1-AP (same clone as 18167-1-PBS)

    WB analysis using 18167-1-AP (same clone as 18167-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 18167-1-AP (AMPK alpha 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18167-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse skeletal muscle using 18167-1-AP (same clone as 18167-1-PBS)

    IP experiment of mouse skeletal muscle using 18167-1-AP (same clone as 18167-1-PBS)

    IP result of anti-AMPK Alpha 2 (IP:18167-1-AP, 4ug; Detection:18167-1-AP 1:1000) with mouse skeletal muscle tissue lysate 3600ug. This data was developed using the same antibody clone with 18167-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 18167-1-AP (same clone as 18167-1-PBS)

    IHC staining of human breast cancer using 18167-1-AP (same clone as 18167-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18167-1-AP (AMPK alpha 2 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18167-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PRKAA2 antibody included
  • IHC staining of human breast cancer using 18167-1-AP (same clone as 18167-1-PBS)

    IHC staining of human breast cancer using 18167-1-AP (same clone as 18167-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18167-1-AP (AMPK alpha 2 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18167-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PRKAA2 antibody included
  • IF Staining of MCF-7 using 18167-1-AP (same clone as 18167-1-PBS)

    IF Staining of MCF-7 using 18167-1-AP (same clone as 18167-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using AMPK Alpha 2 antibody (18167-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 18167-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

AMPK Alpha 2 Polyclonal antibody, PBS Only

AMPK Alpha 2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 18167-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

PRKAA2, 5'-AMP-activated protein kinase catalytic subunit alpha-2, ACACA kinase, Acetyl-CoA carboxylase kinase, AMPK

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy PRKAA2 Ready-To-Use IHC Kit. PRKAA2 primary antibody included.

产品信息

18167-1-PBS targets AMPK Alpha 2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag12796

Product name: Recombinant human PRKAA2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 215-552 aa of BC069680

Sequence: DDEHVPTLFKKIRGGVFYIPEYLNRSVATLLMHMLQVDPLKRATIKDIREHEWFKQDLPSYLFPEDPSYDANVIDDEAVKEVCEKFECTESEVMNSLYSGDPQDQLAVAYHLIIDNRRIMNQASEFYLASSPPSGSFMDDSAMHIPPGLKPHPERMPPLIADSPKARCPLDALNTTKPKSLAVKKAKWHLGIRSQSKPYDIMAEVYRAMKQLDFEWKVVNAYHLRVRRKNPVTGNYVKMSLQLYLVDNRSYLLDFKSIDDEVVEQRSGSSTPQRSCSAAGLHRPRSSFDSTTAESHSLSGSLTGSLTGSTLSSVSPRLGSHTMDFFEMCASLITTLAR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 protein kinase, AMP-activated, alpha 2 catalytic subunit
别名 PRKAA2, 5'-AMP-activated protein kinase catalytic subunit alpha-2, ACACA kinase, Acetyl-CoA carboxylase kinase, AMPK
计算分子量 552 aa, 62 kDa
观测分子量 62 kDa
GenBank蛋白编号BC069680
基因名称 AMPK Alpha 2
Gene ID (NCBI) 5563
RRIDAB_10695046
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP54646
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PRKAA2(protein kinase, AMP-activated, alpha 2 catalytic subunit), also named as AMPKA2, AMPK, PRKAA, AMPK2, belongs to the CAMK Ser/Thr protein kinase family and SNF1 subfamily. PRKAA2 is an αβγ heterotrimer that is activated by low cellular energy status, such as decreases in both the ATP/AMP ratio and the phosphocreatine content and it is a glycogen synthase kinase, phosphorylating Ser7 at the NH2 terminus, which decreases glycogen synthase activity (PMID:14532170). The protein can be ubiquitinated (PMID:21224036).

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