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验证数据展示

  • WB analysis of HEK-293 using 66536-1-Ig (same clone as 66536-1-PBS)

    WB analysis of HEK-293 using 66536-1-Ig (same clone as 66536-1-PBS)

    WB result of AMPK alpha antibody (66536-1-Ig; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-AMPK alpha transfected HEK-293 cells. This data was developed using the same antibody clone with 66536-1-PBS in a different storage buffer formulation.

  • WB analysis using 66536-1-Ig (same clone as 66536-1-PBS)

    WB analysis using 66536-1-Ig (same clone as 66536-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66536-1-Ig (AMPK alpha antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66536-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66536-1-Ig (same clone as 66536-1-PBS)

    IHC staining of human breast cancer using 66536-1-Ig (same clone as 66536-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66536-1-Ig (AMPK alpha antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66536-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PRKAA1 antibody included
  • IHC staining of human breast cancer using 66536-1-Ig (same clone as 66536-1-PBS)

    IHC staining of human breast cancer using 66536-1-Ig (same clone as 66536-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66536-1-Ig (AMPK alpha antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66536-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PRKAA1 antibody included
  • Featured Product
  • KD/KO验证

AMPK Alpha 1 Monoclonal antibody, PBS Only

AMPK Alpha 1 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66536-1-PBS

产品说明书

CloneNo. 1H8E1

宿主/亚型

Mouse / IgG1

种属反应性

Human, Rat, Mouse

应用

WB, IHC, Indirect ELISA

别名

AMPK, AMPK alpha, AMPK Alpha 1, AMPK subunit alpha 1, AMPK1, AMPKa1, PRKAA1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy PRKAA1 Ready-To-Use IHC Kit. PRKAA1 primary antibody included.

产品信息

66536-1-PBS targets AMPK Alpha 1 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, Rat, Mouse samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 Human, Rat, Mouse
免疫原

CatNo: Ag24744

Product name: Recombinant human AMPK alpha protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 1-207 aa of BC012622

Sequence: MATAEKQKHDGRVKIGHYILGDTLGVGTFGKVKVGKHELTGHKVAVKILNRQKIRSLDVVGKIRREIQNLKLFRHPHIIKLYQVISTPSDIFMVMEYVSGGELFDYICKNGRLDEKESRRLFQQILSGVDYCHRHMVVHRDLKPENVLLDAHMNAKIADFGLSNMMSDGEFLRTSCGSPNYAAPEVISGRDCNIIRILTSQFTNYQH

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 protein kinase, AMP-activated, alpha 1 catalytic subunit
别名 AMPK, AMPK alpha, AMPK Alpha 1, AMPK subunit alpha 1, AMPK1, AMPKa1, PRKAA1
计算分子量 63 kDa
观测分子量 64 kDa
GenBank蛋白编号BC012622
基因名称 AMPK Alpha 1
Gene ID (NCBI) 5562
RRIDAB_2881899
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ13131
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The mammalian 5-prime-AMP-activated protein kinase (AMPK) appears to play a role in protecting cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. PRKAA1 is also named as AMPK1, ACACA kinase, HMGCR kinase. It is a mammalian homologue of sucrose non-fermenting protein kinase (SNF-1), which belongs to a serine/threonine protein kinase family. It has 2 isoforms with molecular mass of 63-66 kDa produced by alternative splicing.

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