- Featured Product
- KD/KO Validated
AMPD3 Polyclonal antibody
AMPD3 Polyclonal Antibody for IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IHC, IP, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | RAW 264.7 cells, rat kidney tissue, HEK-293 cells, mouse kidney tissue, mouse lung tissue |
| Positive IP detected in | mouse kidney tissue |
| Positive IHC detected in | human placenta tissue, human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
The immunogen of 23997-1-AP is AMPD3 Fusion Protein expressed in E. coli.
| Tested Applications | IHC, IP, WB, ELISA |
| Cited Applications | WB, IP, IHC |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Immunogen | AMPD3 fusion protein Ag21199 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | adenosine monophosphate deaminase (isoform E) |
| Synonyms | AMP deaminase 3, AMP deaminase isoform E, AMPD3, Erythrocyte AMP deaminase |
| Calculated Molecular Weight | 776 aa, 90 kDa |
| Observed Molecular Weight | 70 kDa, 90 kDa |
| GenBank Accession Number | BC126118 |
| Gene Symbol | AMPD3 |
| Gene ID (NCBI) | 272 |
| RRID | AB_2879396 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen Affinity purified |
| UNIPROT ID | Q01432 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for AMPD3 antibody 23997-1-AP | Download protocol |
| IHC protocol for AMPD3 antibody 23997-1-AP | Download protocol |
| IP protocol for AMPD3 antibody 23997-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Mol Cell Cardiol Translational regulation by miR-301b upregulates AMP deaminase in diabetic hearts. | ||
J Mol Cell Cardiol Xanthine oxidoreductase-mediated injury is amplified by upregulated AMP deaminase in type 2 diabetic rat hearts under the condition of pressure overload. | ||
Nat Metab Super-enhancer-driven metabolic reprogramming promotes cystogenesis in autosomal dominant polycystic kidney disease.
| ||
J Med Chem Discovery of Novel N-(5-(Pyridin-3-yl)-1H-indazol-3-yl)benzamide Derivatives as Potent Cyclin-Dependent Kinase 7 Inhibitors for the Treatment of Autosomal Dominant Polycystic Kidney Disease | ||
Physiol Rep Downregulation of extramitochondrial BCKDH and its uncoupling from AMP deaminase in type 2 diabetic OLETF rat hearts |
