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AMOT Polyclonal antibody

AMOT Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 24550-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, IP, ELISA and More (1)

KIAA1071, Angiomotin

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -


经过测试的应用

Positive WB detected inHEK-293 cells, HEK-293T
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:9000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24550-1-AP targets AMOT in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IF, IP
经测试反应性 human
文献引用反应性human
免疫原 AMOT fusion protein Ag19784 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 angiomotin
别名 KIAA1071, Angiomotin
计算分子量 1084 aa, 118 kDa
观测分子量 80 kDa, 130 kDa
GenBank蛋白编号BC130294
基因名称 AMOT
Gene ID (NCBI) 154796
RRIDAB_2879603
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ4VCS5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Angiomotin belongs to the motion family of angiostatin-binding proteins. The encoded protein is expressed predominantly in endothelial cells of capillaries as well as larger vessels of the placenta where it may mediate the inhibitory effect of angiostatin on tube formation and the migration of endothelial cells during the formation of new blood vessels. The most abundant expression was found in the placenta and skeletal muscle. AMOT has two isoforms with MW 130 kDa (p130) and 80 kDa (p80). The p130 isoform can interact with F-actin. This antibody recognizes both p130 and p80.

实验方案

Product Specific Protocols
WB protocol for AMOT antibody 24550-1-APDownload protocol
IHC protocol for AMOT antibody 24550-1-APDownload protocol
IF protocol for AMOT antibody 24550-1-APDownload protocol
IP protocol for AMOT antibody 24550-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Theranostics

The WW domains dictate isoform-specific regulation of YAP1 stability and pancreatic cancer cell malignancy.

Authors - Qiang Guo
humanIF

Sci Rep

MAGI1 inhibits the AMOTL2/p38 stress pathway and prevents luminal breast tumorigenesis.

Authors - Diala Kantar
humanIP

PLoS One

Regulation of YAP and Wnt signaling by the endosomal protein MAMDC4

Authors - Christopher M Cox
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