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验证数据展示

  • WB analysis using 32038-1-AP

    WB analysis using 32038-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32038-1-AP (AMN antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 32038-1-AP

    IF Staining of HeLa using 32038-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using AMN antibody (32038-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

AMN Polyclonal antibody

AMN Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 32038-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

Protein amnionless, PRO1028, IGS2, Amnionless Homolog, Amnionless

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHuH-7 cells, Jurkat cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

32038-1-AP targets AMN in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag35205

Product name: Recombinant human AMN protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 20-204 aa of NM_030943.3

Sequence: VSKLWVPNTDFDVAANWSQNRTPCAGGAVEFPADKMVSVLVQEGHAVSDMLLPLDGELVLASGAGFGVSDVGSHLDCGAGEPAVFRDSDRFSWHDPHLWRSGDEAPGLFFVDAERVPCRHDDVFFPPSASFRVGLGPGASPVRVRSISALGRTFTRDEDLAVFLASRAGRLRFHGPGALSVGPED

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 amnionless homolog (mouse)
别名 Protein amnionless, PRO1028, IGS2, Amnionless Homolog, Amnionless
计算分子量48 kDa
观测分子量48 kDa
GenBank蛋白编号NM_030943.3
基因名称 AMN
Gene ID (NCBI) 81693
RRIDAB_3670177
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ9BXJ7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Amnion associated transmembrane protein (AMN, also known as IGS2, PRO1028, and amnionless) is a 45-50 kDa cysteine-rich type I transmembrane protein that is expressed exclusively in the extra-embryonic visceral endoderm layer during gastrulation (PMID: 17990981; 11279523). It may direct the production of trunk mesoderm derived from the middle streak by acting in the underlying visceral endoderm to modulate a BMP signaling pathway (PMID: 11279523).

实验方案

Product Specific Protocols
IF protocol for AMN antibody 32038-1-APDownload protocol
WB protocol for AMN antibody 32038-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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