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验证数据展示

  • WB analysis of MCF-7 using 21278-1-AP

    WB analysis of MCF-7 using 21278-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 21278-1-AP (AMIGO3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 21278-1-AP

    IF Staining of MCF-7 using 21278-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 21278-1-AP (AMIGO3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

AMIGO3 Polyclonal antibody

AMIGO3 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 21278-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

Amphoterin induced protein 3, AMIGO-3, AMIGO 3, Alivin-3, Alivin 3

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inMCF7 cells, HeLa cells, human brain tissue, L02 cells, mouse kidney tissue, mouse ovary tissue
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21278-1-AP targets AMIGO3 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag15790

Product name: Recombinant human AMIGO3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 221-504 aa of BC110418

Sequence: LPCDCRLYHLLQRWHQRGLSAVRDFAREYVCLAFKVPASRVRFFQHSRVFENCSSAPALGLERPEEHLYALVGRSLRLYCNTSVPAMRIAWVSPQQELLRAPGSRDGSIAVLADGSLAIGNVQEQHAGLFVCLATGPRLHHNQTHEYNVSVHFPRPEPEAFNTGFTTLLGCAVGLVLVLLYLFAPPCRCCRRACRCRRWPQTPSPLQELSAQSSVLSTTPPDAPSRKASVHKHVVFLEPGRRGLNGRVQLAVAEEFDLYNPGGLQLKAGSESASSIGSEGPMTT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 adhesion molecule with Ig-like domain 3
别名 Amphoterin induced protein 3, AMIGO-3, AMIGO 3, Alivin-3, Alivin 3
计算分子量 504 aa, 55 kDa
观测分子量 45-50 kDa
GenBank蛋白编号BC110418
基因名称 AMIGO3
Gene ID (NCBI) 386724
RRIDAB_10733101
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ86WK7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for AMIGO3 antibody 21278-1-APDownload protocol
WB protocol for AMIGO3 antibody 21278-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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