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AMIGO3 Polyclonal antibody

AMIGO3 Polyclonal Antibody for IF/ICC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, WB,ELISA

Conjugate

Unconjugated

Cat No : 21278-1-AP

Print datasheet

Synonyms

ALI3, Alivin 3, AMIGO 3, AMIGO3, Amphoterin induced protein 3, KIAA1851

验证数据展示

  • WB analysis of MCF-7 using 21278-1-AP

    WB analysis of MCF-7 using 21278-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 21278-1-AP (AMIGO3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 21278-1-AP

    IF Staining of MCF-7 using 21278-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 21278-1-AP (AMIGO3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inMCF7 cells, HeLa cells, human brain tissue, L02 cells, mouse kidney tissue, mouse ovary tissue
Positive IF detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 21278-1-AP is AMIGO3 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, WB,ELISA
Tested Reactivity human, mouse, rat
Immunogen AMIGO3 fusion protein Ag15790 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name adhesion molecule with Ig-like domain 3
Synonyms ALI3, Alivin 3, AMIGO 3, AMIGO3, Amphoterin induced protein 3, KIAA1851
Calculated Molecular Weight 504 aa, 55 kDa
Observed Molecular Weight 45-50 kDa
GenBank Accession NumberBC110418
Gene Symbol AMIGO3
Gene ID (NCBI) 386724
RRIDAB_10733101
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86WK7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for AMIGO3 antibody 21278-1-APDownload protocol
IF protocol for AMIGO3 antibody 21278-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols