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验证数据展示

  • WB analysis of mouse brain using 21874-1-AP

    WB analysis of mouse brain using 21874-1-AP

    mouse brain tissue and rat brain tissue were subjected to SDS PAGE followed by western blot with 21874-1-AP (AMIGO1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

AMIGO1 Polyclonal antibody

AMIGO1 Polyclonal Antibody for WB, ELISA
Cat No. 21874-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA

别名

ALI2, Alivin 2, AMIGO, AMIGO 1, AMIGO1, Amphoterin induced protein 1, KIAA1163

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21874-1-AP targets AMIGO1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 AMIGO1 fusion protein Ag16226 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 adhesion molecule with Ig-like domain 1
别名 ALI2, Alivin 2, AMIGO, AMIGO 1, AMIGO1, Amphoterin induced protein 1, KIAA1163
计算分子量 493 aa, 55 kDa
观测分子量 70-80 kDa
GenBank蛋白编号BC040879
基因名称 AMIGO1
Gene ID (NCBI) 57463
RRIDAB_2878935
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ86WK6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AMIGO1 is a brain-enriched transmembrane immunoglobulin (Ig) superfamily protein with six extracellular leucine-rich repeats (LRR) and a single immunoglobulin-like (Ig) domain (PMID: 21938721). It is widely expressed in the central nervous system (CNS), and can be found in neurons, astrocytes as well as oligodendrocytes. AMIGO1 acts as a homophilic adhesion molecule that induces outgrowth and fasciculation of neurites in central neurons (PMID: 12629050). It has been reported that AMIGO1 is a glycosylated protein, the apparent molecular weight (70-80 kDa) is much higher than the predicted molecular size of AMIGO1 (55 kDa) (PMID: 12629050; 21938721; 22056818).

实验方案

Product Specific Protocols
WB protocol for AMIGO1 antibody 21874-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols