验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells |
| Positive IHC detected in | human prostate cancer tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11052-1-AP targets AMD1 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag1525 Product name: Recombinant human AMD1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-334 aa of BC000171 Sequence: MEAAHFFEGTEKLLEVWFSRQQPDANQGSGDLRTIPRSEWDILLKDVQCSIISVTKTDKQEAYVLSESSMFVSKRRFILKTCGTTLLLKALVPLLKLARDYSGFDSIQSFFYSRKNFMKPSHQGYPHRNFQEEIEFLNAIFPNGAAYCMGRMNSDCWYLYTLDFPESRVISQPDQTLEILMSELDPAVMDQFYMKDGVTAKDVTRESGIRDLIPGSVIDATMFNPCGYSMNGMKSDGTYWTIHITPEPEFSYVSFETNLSQTSYDDLIRKVVEVFKPGKFVTTLFVNQSSKCRTVLASPQKIEGFKRLDCQSAMFNDYNFVFTSFAKKQQQQQS 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | adenosylmethionine decarboxylase 1 |
| 别名 | ADOMETDC, AMD, AMD1, SAMDC |
| 计算分子量 | 38 kDa, 32 kDa |
| 观测分子量 | 38-40 kDa |
| GenBank蛋白编号 | BC000171 |
| 基因名称 | AMD1 |
| Gene ID (NCBI) | 262 |
| RRID | AB_2226430 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P17707 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for AMD1 antibody 11052-1-AP | Download protocol |
| IHC protocol for AMD1 antibody 11052-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nature mTORC1-dependent AMD1 regulation sustains polyamine metabolism in prostate cancer.
| ||
Nucleic Acids Res Genome-wide RNAi screen identifies novel players in human 60S subunit biogenesis including key enzymes of polyamine metabolism. | ||
Clin Transl Med AMD1 upregulates hepatocellular carcinoma cells stemness by FTO mediated mRNA demethylation.
| ||
Breast Cancer Res AMD1 promotes breast cancer aggressiveness via a spermidine-eIF5A hypusination-TCF4 axis | ||
iScience The KRAS-G12D mutation induces metabolic vulnerability in B-cell acute lymphoblastic leukemia. | ||
iScience Linkage of methionine addiction, histone lysine hypermethylation, and malignancy. |
