验证数据展示
经过测试的应用
| Positive WB detected in | MCF-7 cells, HepG2 cells, HT-1080 cells, human brain tissue, mouse brain tissue, U2OS cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells, MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13762-1-AP targets AMBRA1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, CoIP, ELISA |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag4735 Product name: Recombinant human AMBRA1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 906-1208 aa of BC045609 Sequence: NVLYPMPADQRRHVSINSARWLPEPGLGLAYGTNKGDLVICRPEALNSGVEYYWDQLNETVFTVHSNSRSSERPGTSRATWRTDRDMGLMNAIGLQPRNPATSVTSQGTETLALQLQNAETQTEREVPEPGTAASGPGEGEGSEYGASGEDALSRIQRLMAEGGMTAVVQREQSTTMASMGGFGNNIIVSHRIHRSSQTGTEPGAAHTSSPQPSTSRGLLPEAGQLAERGLSPRTASWDQPGTPGREPTQPTLPSSSPVPIPVSLPSAEGPTVHCELTNNNHLLDGGSSRGDAAGPRGEPRNR 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | autophagy/beclin-1 regulator 1 |
| 别名 | Activating molecule in BECN1-regulated autophagy protein 1, DCAF3, DDB1- and CUL4-associated factor 3, KIAA1736, WDR94 |
| 计算分子量 | 1208 aa, 133 kDa |
| 观测分子量 | 110kDa, 130-135 kDa |
| GenBank蛋白编号 | BC045609 |
| 基因名称 | AMBRA1 |
| Gene ID (NCBI) | 55626 |
| RRID | AB_10642007 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9C0C7 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
AMBRA1 (Activating molecule in BECN1-regulated autophagy protein 1), also known as WDR94 or KIAA1736, is a 1,298 amino acid protein that contains three WD repeats. AMBRA1 regulates autophagy and development of the nervous system. Involved in autophagy in controlling protein turnover during neuronal development, and in regulating normal cell survival and proliferation. This antibody works well in WB, IHC and IF application. The full length of Ambra1 is about 140 kDa, with the C-terminal fragment measuring approximately 100 kDa and the N-terminal fragment measuring approximately 50 kDa (PMID: 22441670).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for AMBRA1 antibody 13762-1-AP | Download protocol |
| IHC protocol for AMBRA1 antibody 13762-1-AP | Download protocol |
| WB protocol for AMBRA1 antibody 13762-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun F-box protein FBXO32 ubiquitinates and stabilizes D-type cyclins to drive cancer progression | ||
Adv Sci (Weinh) Redox-Induced Stabilization of AMBRA1 by USP7 Promotes Intestinal Oxidative Stress and Colitis Through Antagonizing DUB3-Mediated NRF2 Deubiquitination
| ||
Adv Sci (Weinh) CDK4/6 Inhibitors Impede Chemoresistance and Inhibit Tumor Growth of Small Cell Lung Cancer
| ||
Cell Death Differ AMBRA1 promotes intestinal inflammation by antagonizing PP4R1/PP4c mediated IKK dephosphorylation in an autophagy-independent manner | ||
Pharmacol Res miR-103a-3p regulates mitophagy in Parkinson's disease through Parkin/Ambra1 signaling. |
