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验证数据展示

  • WB analysis using 84891-9-RR

    WB analysis using 84891-9-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84891-9-RR (AMACR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 84891-9-RR

    IHC staining of human prostate cancer using 84891-9-RR

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 84891-9-RR (AMACR antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human prostate cancer using 84891-9-RR

    IHC staining of human prostate cancer using 84891-9-RR

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 84891-9-RR (AMACR antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of LNCaP using 84891-9-RR

    IF Staining of LNCaP using 84891-9-RR

    Immunofluorescent analysis of (4% PFA) fixed LNCaP cells using AMACR antibody (84891-9-RR, Clone: 250503C12 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84891-9-RR

    Affinity and Kinetic Characterization of 84891-9-RR

    Biolayer interferometry (BLl) kinetic assays of 84891-9-RR against Human AMACR were performed. The affinity constant is 20.0 nM.

AMACR/p504S Recombinant monoclonal antibody

AMACR/p504S Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 84891-9-RR

产品说明书

CloneNo. 250503C12

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

AMACR, p504S, 2-methylacyl-CoA racemase, Alpha-methylacyl-CoA racemase, EC:5.1.99.4

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHepG2 cells, MCF-7 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inLNCaP cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84891-9-RR targets AMACR/p504S in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Eg4005

Product name: Recombinant Human AMACR/p504S protein (rFc Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 200-330 aa of NM_014324.6

Sequence: KLSLWEAPRGQNMLDGGAPFYTTYRTADGEFMAVGAIEPQFYELLIKGLGLKSDELPNQMSMDDWPEMKKKFADVFAEKTKAEWCQIFDGTDACVTPVLTFEEVVHHDHNKERGSFITSEEQDVSPRPAPL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 alpha-methylacyl-CoA racemase
别名 AMACR, p504S, 2-methylacyl-CoA racemase, Alpha-methylacyl-CoA racemase, EC:5.1.99.4
计算分子量42 kDa
观测分子量42 kDa
GenBank蛋白编号NM_014324.6
基因名称 AMACR
Gene ID (NCBI) 23600
RRIDAB_3743818
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9UHK6-1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AMACR(Alpha-methyl acyl-CoA racemase) belongs to the CaiB/BaiF CoA-transferase family. A mitochondrial and peroxisomal enzyme catalyzes the conversion of 2R stereoisomers of phytanic and pristanic acid to their S counterparts. AMACR has previously been shown to be a highly sensitive marker for colorectal and clinically localized prostate cancer (PCa). However, AMACR expression is down-regulated at the transcript and protein level in hormone-refractory metastatic PCa, suggesting a hormone-dependent expression of AMACR(PMID:12213712). It has 3 isoforms produced by alternative splicing and can form a dimer of 70-75 kDa(PMID:21812041). Defects in AMACR cause alpha-methyl acyl-CoA racemase deficiency (AMACRD) and congenital bile acid synthesis defect type 4 (CBAS4).

实验方案

Product Specific Protocols
IF protocol for AMACR/p504S antibody 84891-9-RRDownload protocol
IHC protocol for AMACR/p504S antibody 84891-9-RRDownload protocol
WB protocol for AMACR/p504S antibody 84891-9-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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