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验证数据展示

  • WB analysis using 13998-1-AP

    WB analysis using 13998-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13998-1-AP (ALS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of HEK-293 using 13998-1-AP

    IP experiment of HEK-293 using 13998-1-AP

    IP result of anti-ALS2 (IP:13998-1-AP, 4ug; Detection:13998-1-AP 1:800) with HEK-293 cells lysate 1920 ug.

  • IHC staining of mouse cerebellum using 13998-1-AP

    IHC staining of mouse cerebellum using 13998-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 13998-1-AP (ALS2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ALS2 antibody included

ALS2 Polyclonal antibody

ALS2 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 13998-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, ELISA

别名

ALS2CR6, Alsin, ALSJ, IAHSP, PLSJ

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse cerebellum tissue, HEK-293 cells, rat cerebellum tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy ALS2 Ready-To-Use IHC Kit. ALS2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13998-1-AP targets ALS2 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag5087

Product name: Recombinant human ALS2 protein

Source: e coli.-derived, T-HIS

Tag: 6*His

Domain: 1-304 aa of BC029174

Sequence: MDSKKRSSTEAEGSKERGLVHIWQAGSFPITPERLPGWGGKTVLQAALGVKHGVLLTEDGEVYSFGTLPWRSGPVEICPSSPILENALVGQYVITVATGSFHSGAVTDNGVAYMCGENSAGQCAVANQQYVPEPNPVSIADSEASPLLAVRILQLACGEEHTLALSISREIWAWGTGCQLGLITTAFPVTKPQKVEHLAGRVVLQVACGAFHSLALVQCLPSQDLKPVPERCNQCSQLLITMTDKEDHVIISDSHCCPLGVTLTESQAENHASTALSPSTETLDRQEEVFENTLVANDQSVATE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 amyotrophic lateral sclerosis 2 (juvenile)
别名 ALS2CR6, Alsin, ALSJ, IAHSP, PLSJ
计算分子量 1657 aa, 184 kDa
观测分子量 185 kDa
GenBank蛋白编号BC029174
基因名称 ALS2
Gene ID (NCBI) 57679
RRIDAB_10858476
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96Q42
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ALS2 antibody 13998-1-APDownload protocol
IHC protocol for ALS2 antibody 13998-1-APDownload protocol
IP protocol for ALS2 antibody 13998-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols