15 Lipoxygenase 2 Polyclonal antibody

15 Lipoxygenase 2 Polyclonal Antibody for WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB,ELISA and More (1)

Conjugate

Unconjugated

Cat No : 13073-1-AP

Print datasheet

Synonyms

15 Lipoxygenase 2, 15 LOX 2, 15 LOX B, ALOX15B, ALOX8, Arachidonate 15 lipoxygenase B



经过测试的应用

Positive WB detected inA431 cells, C2C12 cells, HEK-293 cells, HL-60 cells, HeLa cells, MCF-7 cells, MCF7 cells, mouse skeletal muscle tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Sample-dependent, check data in validation data gallery

产品信息

13073-1-AP targets 15 Lipoxygenase 2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB,ELISA
Cited Applications IHC, WB
Tested Reactivity human, mouse
Cited Reactivitymouse, rat, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen 15 Lipoxygenase 2 fusion protein Ag3741 种属同源性预测
Full Name arachidonate 15-lipoxygenase, type B
Synonyms 15 Lipoxygenase 2, 15 LOX 2, 15 LOX B, ALOX15B, ALOX8, Arachidonate 15 lipoxygenase B
Calculated molecular weight 676 aa, 76 kDa
Observed molecular weight 67-76 kDa
GenBank accession numberBC035217
Gene symbol ALOX15B
UniprotO15296
Gene ID (NCBI) 247
RRIDAB_2227031
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

In humans, there are 6 functional lipoxygenases genes. Two of them encode for arachidonic acid 12-lipoxygenating (ALOX12, ALOX12B) and two for 15-lipoxygenating enzymes (ALOX15, ALOX15B). ALOX15B has been described as the most abundant 15-lipoxygenating enzyme species in human carotid atherosclerotic lesions and to be associated with cerebrovascular symptoms. (PMID:24373925). ALOX15B has 4 isoforms produced by alternative splicing with the MW of 76, 67, 69 and 73 kDa. 13073-1-AP can also recognize the mouse Alox8 protein.

实验方案

Product Specific Protocols
WB protocol for 15 Lipoxygenase 2 antibody 13073-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratIHC

Exp Cell Res

Lipoxin A4 and its analog attenuate high fat diet-induced atherosclerosis via Keap1/Nrf2 pathway.

Authors - Fen Xu
mouseWB

J Chem Neuroanat

Proanthocyanidin promotes functional recovery of spinal cord injury via inhibiting ferroptosis.

Authors - Huangao Zhou
pigWB

Theriogenology

The role of ALOX15B in heat stress-induced apoptosis of porcine sertoli cells.

Authors - Hongyan Xue