|Positive WB detected in||A431 cells, C2C12 cells, HEK-293 cells, HL-60 cells, HeLa cells, MCF-7 cells, MCF7 cells, mouse skeletal muscle tissue|
|Western Blot (WB)||WB : 1:500-1:3000|
|Sample-dependent, check data in validation data gallery|
13073-1-AP targets 15 Lipoxygenase 2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
|Cited Applications||IHC, WB|
|Tested Reactivity||human, mouse|
|Cited Reactivity||mouse, rat, pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||15 Lipoxygenase 2 fusion protein Ag3741|
|Full Name||arachidonate 15-lipoxygenase, type B|
|Synonyms||15 Lipoxygenase 2, 15 LOX 2, 15 LOX B, ALOX15B, ALOX8, Arachidonate 15 lipoxygenase B|
|Calculated molecular weight||676 aa, 76 kDa|
|Observed molecular weight||67-76 kDa|
|GenBank accession number||BC035217|
|Gene ID (NCBI)||247|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
In humans, there are 6 functional lipoxygenases genes. Two of them encode for arachidonic acid 12-lipoxygenating (ALOX12, ALOX12B) and two for 15-lipoxygenating enzymes (ALOX15, ALOX15B). ALOX15B has been described as the most abundant 15-lipoxygenating enzyme species in human carotid atherosclerotic lesions and to be associated with cerebrovascular symptoms. (PMID:24373925). ALOX15B has 4 isoforms produced by alternative splicing with the MW of 76, 67, 69 and 73 kDa. 13073-1-AP can also recognize the mouse Alox8 protein.
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