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验证数据展示

  • IHC staining of rat kidney using 25566-1-AP

    IHC staining of rat kidney using 25566-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 25566-1-AP (ALKBH8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HEK-293 using 25566-1-AP

    IF Staining of HEK-293 using 25566-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using ALKBH8 antibody (25566-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IF Staining of U2OS using 25566-1-AP

    IF Staining of U2OS using 25566-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using ALKBH8 antibody (25566-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

ALKBH8 Polyclonal antibody

ALKBH8 Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 25566-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

IHC, IF/ICC, ELISA

别名

ABH8, EC:2.1.1.229, Probable alpha-ketoglutarate-dependent dioxygenase ABH8, S-adenosyl-L-methionine-dependent tRNA methyltransferase ABH8, tRNA (carboxymethyluridine(34)-5-O)-methyltransferase ABH8

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inrat kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells, HEK-293 cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25566-1-AP targets ALKBH8 in IHC, IF/ICC, ELISA applications and shows reactivity with human, rat samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
经测试反应性 human, rat
免疫原

CatNo: Ag22239

Product name: Recombinant human ALKBH8 protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 1-238 aa of BC015183

Sequence: MDSNHQSNYKLSKTEKKFLRKQIKAKHTLLRHEGIETVSYATQSLVVANGGLGNGVSRNQLLPVLEKCGLVDALLMPPNKPYSFARYRTTEESKRAYVTLNGKEVVDDLGQKITLYLNFVEKVQWKELRPQALPPGLMVVEEIISSEEEKMLLESVDWTEDTDNQNSQKSLKHRRVKHFGYEFHYENNNVDKDKPLSGGLPDICESFLEKWLRKGYIKHKPDQMTINQYEPGQDCHGF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 alkB, alkylation repair homolog 8 (E. coli)
别名 ABH8, EC:2.1.1.229, Probable alpha-ketoglutarate-dependent dioxygenase ABH8, S-adenosyl-L-methionine-dependent tRNA methyltransferase ABH8, tRNA (carboxymethyluridine(34)-5-O)-methyltransferase ABH8
计算分子量 664 aa, 75 kDa
GenBank蛋白编号BC015183
基因名称 ALKBH8
Gene ID (NCBI) 91801
RRIDAB_3742152
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96BT7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ALKBH8 (Alpha-ketoglutarate-dependent dioxygenase) is unique among the ALKBH family, since it harbors a 2-oxoglutarate and Fe(II)-dependent oxygenase domain as well as an RNA recognition motif and methyl transferase domain (PMID: 27329810). ALKBH8 is a "writer" enzyme that modifies the wobble uridine of tRNA and regulates protein translation, including that of selenoproteins (PMID: 38550277). ALKBH8 is highly expressed in high-grade, superficially and deeply invasive bladder cancer. ALKBH8 knockdown induces apoptosis in bladder cancer cells (PMID: 38550277).

实验方案

Product Specific Protocols
IF protocol for ALKBH8 antibody 25566-1-APDownload protocol
IHC protocol for ALKBH8 antibody 25566-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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