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ALKBH7 Polyclonal antibody

ALKBH7 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 15470-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

ABH7, ALKBH7, SPATA11, UNQ6002

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存


经过测试的应用

Positive WB detected inhuman testis tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15470-1-AP targets ALKBH7 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原

CatNo: Ag7772

Product name: Recombinant human ALKBH7 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-221 aa of BC004393

Sequence: MAGTGLLALRTLPGPSWVRGSGPSVLSRLQDAAVVRPGFLSTAEEETLSRELEPELRRRRYEYDHWDAAIHGFRETEKSRWSEASRAILQRVQAAAFGPGQTLLSSVHVLDLEARGYIKPHVDSIKFCGATIAGLSLLSPSVMRLVHTQEPGEWLELLLEPGSLYILRGSARYDFSHEILRDEESFFGERRIPRGRRISVICRSLPEGMGPGESGQPPPAC

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 alkB, alkylation repair homolog 7 (E. coli)
别名 ABH7, ALKBH7, SPATA11, UNQ6002
计算分子量 25 kDa
观测分子量 25 kDa
GenBank蛋白编号BC004393
基因名称 ALKBH7
Gene ID (NCBI) 84266
RRIDAB_2226855
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BT30
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ALKBH7(Alkylated DNA repair protein alkB homolog 7) is also named as ABH7, SPATA11 and belongs to the alkB family. This protein may be a dioxygenase that requires molecular oxygen, alpha-ketoglutarate and iron. Quantitative real-time PCR detectes variable ALKBH7 expression in all 16 tissues examined, with highest expression in pancreas, followed by spleen, prostate, ovary, and placenta(PMID:17979886). The gene encodes a 25 kDa protein with a 20 amino acid signal peptide.

实验方案

Product Specific Protocols
WB protocol for ALKBH7 antibody 15470-1-APDownload protocol
IHC protocol for ALKBH7 antibody 15470-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Cell Biol

ALKBH7-mediated demethylation regulates mitochondrial polycistronic RNA processing.

Authors - Li-Sheng Zhang
  • KD Validated
human,mouseWB

Cell Death Dis

ALKBH7 drives a tissue and sex-specific necrotic cell death response following alkylation-induced damage.

Authors - Jennifer J Jordan
  • KO Validated
humanWB

Cell

A glycolytic metabolite bypasses "two-hit" tumor suppression by BRCA2

Authors - Li Ren Kong
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