验证数据展示
经过测试的应用
| Positive WB detected in | mouse kidney tissue, MCF-7 cells, K-562 cells, mouse ovary tissue |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
19882-1-AP targets ALKBH4 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, pig |
| 免疫原 |
CatNo: Ag8041 Product name: Recombinant human ALKBH4 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-302 aa of BC002820 Sequence: MAAAAAETPEVLRECGCKGIRTCLICERQRGSDPPWELPPAKTYRFIYCSDTGWAVGTEESDFEGWAFPFPGVMLIEDFVTREEEAELVRLMDRDPWKLSQSGRRKQDYGPKVNFRKQKLKTEGFCGLPSFSREVVRRMGLYPGLEGFRPVEQCNLDYCPERGSAIDPHLDDAWLWGERLVSLNLLSPTVLSMCREAPGSLLLCSAPSAAPEALVDSVIAPSRSVLCQEVEVAIPLPARSLLVLTGAARHQWKHAIHRRHIEARRVCVTFRELSAEFGPGGRQQELGQELLRIALSFQGRPV 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | alkB, alkylation repair homolog 4 (E. coli) |
| 别名 | ABH4, Alpha-ketoglutarate-dependent dioxygenase alkB homolog 4, DNA N6-methyl adenine demethylase ALKBH4, EC:1.14.11.-, EC:1.14.11.51 |
| 计算分子量 | 34 kDa |
| 观测分子量 | 34 kDa |
| GenBank蛋白编号 | BC002820 |
| 基因名称 | ALKBH4 |
| Gene ID (NCBI) | 54784 |
| RRID | AB_10792931 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9NXW9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for ALKBH4 antibody 19882-1-AP | Download protocol |
| WB protocol for ALKBH4 antibody 19882-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cancer Discov Identification of BBOX1 as a Therapeutic Target in Triple-Negative Breast Cancer.
| ||
Sci Rep ALKBH overexpression in head and neck cancer: potential target for novel anticancer therapy.
| ||
Water (Basel) ALKBH4 Stabilization Is Required for Arsenic-Induced 6mA DNA Methylation Inhibition, Keratinocyte Malignant Transformation, and Tumorigenicity
| ||
Sci Rep The proteomic profile is altered but not repaired after bariatric surgery in type 2 diabetes pigs |
