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验证数据展示

  • WB analysis of HeLa using 28084-1-AP (same clone as 28084-1-PBS)

    WB analysis of HeLa using 28084-1-AP (same clone as 28084-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 28084-1-AP (ALG3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28084-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse placenta using 28084-1-AP (same clone as 28084-1-PBS)

    IHC staining of mouse placenta using 28084-1-AP (same clone as 28084-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 28084-1-AP (ALG3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28084-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ALG3 antibody included
  • IHC staining of mouse placenta using 28084-1-AP (same clone as 28084-1-PBS)

    IHC staining of mouse placenta using 28084-1-AP (same clone as 28084-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 28084-1-AP (ALG3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28084-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ALG3 antibody included

ALG3 Polyclonal antibody, PBS Only

ALG3 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 28084-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy ALG3 Ready-To-Use IHC Kit. ALG3 primary antibody included.

产品信息

28084-1-PBS targets ALG3 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag26382

Product name: Recombinant human ALG3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-199 aa of BC002839

Sequence: MAAGLRKRGRSGSAAQAEGLCKQWLQRAWQERRLLLREPRYTLLVAACLCLAEVGITFWVIHRVAYTEIDWKAYMAEVEGVINGTYDYTQLQGDTGPLVYPAGFVYIFMGLYYATSRGTDIRMAQNIFAVLYLATLLLVFLIYHQTCKVPPFVFFFMCCASYRVHSIFVLRLFNDPVAMVLLFLSINLLLAQRWGWGCC

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 asparagine-linked glycosylation 3, alpha-1,3- mannosyltransferase homolog (S. cerevisiae)
别名
计算分子量 438 aa, 50 kDa
观测分子量 50-60 kDa
GenBank蛋白编号BC002839
基因名称 ALG3
Gene ID (NCBI) 10195
RRIDAB_3669644
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ92685
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Alpha-1,3-mannosyltransferase (ALG3) encodes an endoplasmic reticulum-localized ALG3 family member that participates in N-glycan synthesis. ALG3 contributes to high mannose-type N-glycans in several cancers, and mannose-type N-glycan upregulation has been shown to be related to cancer progression. ALG3 is overexpressed in oral squamous cell carcinoma(PMID: 33084111), non-small cell lung cancer(PMID: 31899049), and breast cancer(PMID: 33931075) and hepatocellular carcinoma(PMID: 35782861).

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