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验证数据展示

  • WB analysis of HeLa using 12872-1-AP (same clone as 12872-1-PBS)

    WB analysis of HeLa using 12872-1-AP (same clone as 12872-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 12872-1-AP (ALG1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12872-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 12872-1-AP (same clone as 12872-1-PBS)

    IP experiment of HeLa using 12872-1-AP (same clone as 12872-1-PBS)

    IP result of anti-ALG1 (IP:12872-1-AP, 4ug; Detection:12872-1-AP 1:500) with HeLa cells lysate 3080 ug. This data was developed using the same antibody clone with 12872-1-PBS in a different storage buffer formulation.

  • IHC staining of human gliomas using 12872-1-AP (same clone as 12872-1-PBS)

    IHC staining of human gliomas using 12872-1-AP (same clone as 12872-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12872-1-AP (ALG1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12872-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ALG1 antibody included
  • IHC staining of human gliomas using 12872-1-AP (same clone as 12872-1-PBS)

    IHC staining of human gliomas using 12872-1-AP (same clone as 12872-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12872-1-AP (ALG1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12872-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ALG1 antibody included
  • IF Staining of HeLa using 12872-1-AP (same clone as 12872-1-PBS)

    IF Staining of HeLa using 12872-1-AP (same clone as 12872-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 12872-1-AP (ALG1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12872-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary cancer using 12872-1-AP (same clone as 12872-1-PBS)

    IHC staining of human ovary cancer using 12872-1-AP (same clone as 12872-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 12872-1-AP (ALG1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12872-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ALG1 antibody included
  • IHC staining of human placenta using 12872-1-AP (same clone as 12872-1-PBS)

    IHC staining of human placenta using 12872-1-AP (same clone as 12872-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 12872-1-AP (ALG1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12872-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ALG1 antibody included
  • Featured Product
  • KD/KO Validated

ALG1 Polyclonal antibody, PBS Only

ALG1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 12872-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

Asparagine-linked glycosylation protein 1 homolog, Beta 1,4 mannosyltransferase, Beta-1,4-mannosyltransferase, Chitobiosyldiphosphodolichol beta-mannosyltransferase, EC:2.4.1.142

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy ALG1 Ready-To-Use IHC Kit. ALG1 primary antibody included.

产品信息

12872-1-PBS targets ALG1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag3540

Product name: Recombinant human ALG1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 157-464 aa of BC031095

Sequence: HNYGYSIMGLVHGPNHPLVLLAKWYEKFFGRLSHLNLCVTNAMREDLADNWHIRAVTVYDKPASFFKETPLDLQHRLFMKLGSMHSPFRARSEPEDPVTERSAFTERDAGSGLVTRLRERPALLVSSTSWTEDEDFSILLAALEKFEQLTLDGHNLPSLVCVITGKGPMREYYSRLIHQKHFQHIQVCTPWLEAEDYPLLLGSADLGVCLHTSSSGLDLPMKVVDMFGCCLPVCAVNFKCLHELVKHEENGLVFEDSEELAAQLQMLFSNFPDPAGKLNQFRKNLRESQQLRWDESWVRTVLPLVMDT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 asparagine-linked glycosylation 1, beta-1,4-mannosyltransferase homolog (S. cerevisiae)
别名 Asparagine-linked glycosylation protein 1 homolog, Beta 1,4 mannosyltransferase, Beta-1,4-mannosyltransferase, Chitobiosyldiphosphodolichol beta-mannosyltransferase, EC:2.4.1.142
计算分子量 464 aa, 53 kDa
观测分子量 47-53 kDa
GenBank蛋白编号BC031095
基因名称 ALG1
Gene ID (NCBI) 56052
RRIDAB_2289541
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BT22
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ALG1 (asparagine-linked glycosylation 1) is a β-1,4-mannosyltransferase that catalyzes the committed step of N-linked glycosylation. Operating on the cytoplasmic face of the endoplasmic reticulum, ALG1 transfers the first mannose from GDP-mannose onto dolichol-pyrophosphate-GlcNAc₂, yielding Man₁GlcNAc₂-PP-dolichol. ALG1 is a 464-amino-acid protein with an N-terminal transmembrane domain that is essential for catalytic activity; deleting this domain abolishes mannosyltransferase function.

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